| Host | Rabbit |
| Antigen | SCGN |
| Synonyms | Secretagogin, SECRE |
| Immunogen | Synthetic Peptide |
| Accession | O76038 |
| Clone Number | SDT-040-69 |
| Antibody Type | Rabbit mAb |
| Application | IHC-P, IF |
| Reactivity | Hu, Ms |
| Predicted Reactivity | Bv, Rt, Pg |
| Purification | Protein A |
| Research Area | Neuroscience |
| Concentration | 0.5mg/ml |
| Molecular Weight | 32kDa |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
| application | dilution | species |
| IHC-P | 1:2000 | |
| IF | 1:1000 |
Secretin (SCGN) is a cytoplasmic calcium binding protein, which has homology with calcium binding protein-D28k (CALB1) and calcium reticulum protein (CALB2). Secretin protein was isolated from Langerhans Island and insulinoma cells, and was highly expressed in pancreas, but decreased in thyroid, adrenal medulla, stomach, small intestine and colon. The cloning and sequence analysis of secretin cDNA showed that the corresponding protein included six EF hand calcium binding domain tandem repeats. The interaction between secretin and vesicular transporters (including SNAP25, SNAP23, ARFGAP2 and DOC2A) suggests that SCGN may play a role in the release of neurotransmitters and hormones. The study on the expression of secretin in fetal brain and developing brain shows that this calcium binding protein plays a role in differentiation, brain wiring and neuronal development.
IHC shows positive staining in paraffin-embedded human pancreas. Anti-SCGN antibody was used at 1/2000 dilution, followed by a Goat.
Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon.
Anti-SCGN antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows negative staining in paraffin-embedded human kidney(negative tissues).
Anti-SCGN antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows negative staining in paraffin-embedded human hepatocellular carcinoma(negative tissues).
Anti-SCGN antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse pancreas.
Anti-SCGN antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IF shows positive staining in paraffin-embedded mouse pancreas. Anti-SCGN antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
