SCD Recombinant Rabbit mAb (S-4467-1)

Catalog Number: S0B60683 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	SCD
Synonyms	Stearoyl-CoA desaturase; Acyl-CoA desaturase; Delta(9)-desaturase (Delta-9 desaturase); Fatty acid desaturase; FADS5; SCD1; SCDOS
Immunogen	Recombinant Protein
Location	Endoplasmic reticulum
Accession	O00767
Clone Number	S-4467-1
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:5000	Hu, Ms, Rt
IHC-P	1:2000	Hu, Ms, Rt

Background

SCD (stearoyl-CoA desaturase) is an integral membrane protein localized to the endoplasmic reticulum membrane and is the rate-limiting enzyme in the biosynthesis of monounsaturated fatty acids (MUFAs), catalyzing the introduction of the first cis double bond at the Δ9 position of saturated fatty acyl-CoAs. Humans possess two homologous proteins, SCD1 and SCD5, whereas mice express four isoforms, SCD1 through SCD4. The enzyme uses palmitoyl-CoA and stearoyl-CoA as its principal substrates, converting them to palmitoleoyl-CoA (16:1) and oleoyl-CoA (18:1), respectively, and these monounsaturated fatty acids are key precursors for the synthesis of membrane phospholipids, cholesteryl esters, and triglycerides. The catalytic mechanism of SCD depends on a di-iron center coordinated by nine conserved histidine residues, which activates molecular oxygen, and it also requires cytochrome b5 as an electron donor to regenerate the iron center. Structural biology studies have revealed that SCD1 adopts a unique fold: four transmembrane helices form a conical structure capped by a cytosolic domain, with the substrate acyl chain enclosed within a narrow tunnel approximately 24 Å long that contains a sharp bend at the C9 and C10 positions, thereby precisely determining the positional selectivity and stereospecificity of the desaturation reaction. Functionally, SCD1 knockout mice exhibit a lean, hypermetabolic, insulin-sensitive phenotype with resistance to diet-induced obesity, making SCD an attractive drug target for metabolic diseases such as obesity, diabetes, and non-alcoholic fatty liver disease. At the same time, SCD is overexpressed in a variety of malignant tumors, supporting tumor growth by supplying monounsaturated fatty acids required for membrane biosynthesis and by participating in the lipidation of Wnt signaling pathway ligands, making it a highly watched target in the field of cancer therapy as well.

Picture

Western Blot

WB result of SCD Recombinant Rabbit mAb
Primary antibody: SCD Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled MCF7 whole cell lysate 20 µg
Lane 2: unboiled Hep G2 whole cell lysate 20 µg
Lane 3: unboiled HEK-293 whole cell lysate 20 µg
Lane 4: unboiled SK-MEL-28 whole cell lysate 20 µg
Lane 5: unboiled HeLa whole cell lysate 20 µg
Lane 6: unboiled A-431 whole cell lysate 20 µg
Lane 7: unboiled A-375 whole cell lysate 20 µg
Lane 8: unboiled A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 26, 37 kDa

WB result of SCD Recombinant Rabbit mAb
Primary antibody: SCD Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled differentiated 3T3-L1 whole cell lysate 20 µg
Lane 2: unboiled mouse liver lysate 20 µg
Lane 3: unboiled mouse brown fat lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 37 kDa

WB result of SCD Recombinant Rabbit mAb
Primary antibody: SCD Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled Rat liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 37 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human liver. Anti-SCD antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver cancer. Anti-SCD antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-SCD antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-SCD antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-SCD antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human stomach cancer. Anti-SCD antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse adipose tissue. Anti-SCD antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.