WB result of SCARB2/LIMPII Recombinant Rabbit mAb
Primary antibody: SCARB2/LIMPII Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: SH-SY5Y whole cell lysate 20 µg
Lane 2: HT-29 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 85 kDa
SCARB2/LIMPII Recombinant Rabbit mAb (S-2847-21)
SCARB2/LIMPII Recombinant Rabbit mAb (S-2847-21)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SCARB2/LIMPII |
| Synonyms | Lysosome membrane protein 2; 85 kDa lysosomal membrane sialoglycoprotein (LGP85); CD36 antigen-like 2; Lysosome membrane protein II (LIMP II); Scavenger receptor class B member 2; CD36; CD36L2; LIMP2 |
| Immunogen | Synthetic Peptide |
| Location | Lysosome |
| Accession | Q14108 |
| Clone Number | S-2847-21 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Mk |
| Positive Sample | SH-SY5Y, HT-29, HeLa, HepG2, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Mk |
| IHC-P | 1:1000 | Hu |
| ICC | 1:100 | Hu |
Background
SCARB2/LIMPII, also called lysosomal integral membrane protein-2 (LIMP-2), is an ~85 kDa heavily glycosylated type-III multi-pass membrane protein that resides predominantly in the limiting membranes of lysosomes and late endosomes in every mammalian tissue, where it functions as the mannose-6-phosphate-independent trafficking receptor that escorts the lipid-catabolizing enzyme β-glucocerebrosidase (GBA) from the Golgi to the lysosome lumen, and also serves as the primary cell-surface receptor for enterovirus 71 and several coxsackieviruses that cause hand-foot-and-mouth disease, while genetic loss-of-function mutations in SCARB2 produce the autosomal-recessive “action myoclonus–renal failure syndrome” characterized by progressive myoclonic epilepsy, renal glomerulosclerosis, deafness and peripheral neuropathy.
Picture
Picture
Western Blot
WB result of SCARB2/LIMPII Recombinant Rabbit mAb
Primary antibody: SCARB2/LIMPII Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 85 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-SCARB2/LIMPII antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-SCARB2/LIMPII antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-SCARB2/LIMPII antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in SH-SY5Y cells. Anti-SCARB2/LIMPII antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
