WB result of SCAMP1 Rabbit pAb
Primary antibody: SCAMP1 Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: SH-SY5Y whole cell lysate 20 µg
Lane 3: U-87 MG whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 38 kDa
Observed MW: 35 kDa
SCAMP1 Rabbit Polyclonal Antibody
SCAMP1 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SCAMP1 |
| Synonyms | Secretory carrier-associated membrane protein 1; Secretory carrier membrane protein 1; SCAMP |
| Immunogen | Recombinant Protein |
| Location | Endosome |
| Accession | O15126 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, SH-SY5Y, U-87 MG, MCF7, Neuro-2a, mouse brain, mouse pancreas, rat brain |
| Predicted Reactivity | Pg |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:200 | Hu, Ms, Rt |
Background
SCAMP1 (Secretory Carrier Membrane Protein 1) is a ubiquitously expressed, four-pass transmembrane protein that cycles between the trans-Golgi network, endosomes and the plasma membrane, where it acts as a multifunctional regulator of post-Golgi membrane trafficking, exocytosis and cell-surface recycling; its cytosolic N- and C-terminal domains contain NPF motifs, a proline-rich SH3-binding site and three conserved amphiphilic segments that dock at the cytoplasmic membrane interface, enabling SCAMP1 to nucleate protein–protein interactions with SNAREs and other trafficking factors, thereby facilitating secretory granule exocytosis in neuroendocrine cells and insulin-secreting β-cells, while in epithelia it cooperates with the metastasis suppressor MTSS1 to stabilize Rac1-based cell–cell adhesions and curb cancer invasion, and palmitoylation of its cytosolic segments dynamically modulates these membrane exchange and anti-invasive functions.
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Western Blot
WB result of SCAMP1 Rabbit pAb
Primary antibody: SCAMP1 Rabbit pAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Lane 3: mouse pancreas lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 38 kDa
Observed MW: 35 kDa
WB result of SCAMP1 Rabbit pAb
Primary antibody: SCAMP1 Rabbit pAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 38 kDa
Observed MW: 35 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-SCAMP1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-SCAMP1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-SCAMP1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-SCAMP1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-SCAMP1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
