SC35 Recombinant Mouse mAb (S-5700)

Catalog Number: S0B60682 Application: IHC-P, ICC Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Mouse
Antigen	SC35
Synonyms	Serine/arginine-rich splicing factor 2; Protein PR264; Splicing component; 35 kDa; Splicing factor SC35 (SC-35); Splicing factor; arginine/serine-rich 2; SFRS2; SRSF2
Location	Nucleus
Accession	Q01130
Clone Number	S-5700
Antibody Type	Recombinant mAb
Isotype	IgG1,k
Application	IHC-P, ICC
Reactivity	Hu, Ms, Rt
Purification	Protein A
Concentration	2 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
IHC-P	1:500-1:2000	Hu, Ms, Rt
ICC	1:500	Hu, Ms

Background

SC35, officially known as serine/arginine-rich splicing factor 2 (SRSF2), is a prototype member of the SR protein family encoded by the SRSF2 gene and a key regulator of precursor mRNA splicing. The protein derives its name from its migration at 35 kDa on SDS-PAGE and was first identified in 1992 as a mammalian splicing factor. Structurally, SC35 contains an N-terminal RNA recognition motif (RRM) and a C-terminal arginine/serine-rich (RS) domain: the RRM is responsible for specifically recognizing the 5'-SSNG-3' sequence (S = C/G), while the RS domain regulates its activity through phosphorylation and mediates protein-protein interactions with other spliceosomal components. Functionally, SC35 is required for the formation of early ATP-dependent complexes during spliceosome assembly, bridging U1 snRNP with U2AF to connect the 5' and 3' splice sites. In addition, SC35 participates in the regulation of transcriptional elongation by directly or indirectly mediating the recruitment of elongation factors to the C-terminal domain of RNA polymerase II. The subcellular localization of SC35 is highly characteristic: it is predominantly concentrated in nuclear speckles (also known as SC35 domains), which are not inert storage sites for factors but rather active hubs that spatially link the expression of specific precursor mRNAs with the rapid cycling of RNA metabolic complexes. Notably, SC35 itself is considered a core structural component required for maintaining the integrity of nuclear speckles—its dispersion simultaneously leads to the dispersion of poly(A) RNA, whereas other SR proteins such as ASF/SF2 can be dispersed while SC35 remains structurally intact. In terms of clinical relevance, mutations in SRSF2 are closely associated with a variety of diseases, including myelodysplastic syndromes (MDS), acute myeloid leukemia, hepatocellular carcinoma, and heart failure.

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Immunohistochemistry

IHC shows positive staining in paraffin-embedded human testis. Anti-SC35 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human bladder cancer. Anti-SC35 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-SC35 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-SC35 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-SC35 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse liver. Anti-SC35 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat brain. Anti-SC35 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunocytochemistry

ICC shows positive staining in MCF7 cells. Anti-SC35 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

ICC shows positive staining in NIH/3T3 cells. Anti-SC35 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).