SARS-CoV-2 Omicron RBD (BA.2.75), His tag

Catalog Number: S0A2028 Brand: Starter

Price:

$85.00 USD

Size: 25μg 50μg 100μg

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Product Details

Product Specification

Species	SARS-CoV-2
Accession	P0DTC2
Amino Acid Sequence	Protein sequence (P0DTC2, Arg319-Lys537, with C-10*His) RVQPTESIVRFPNITNLCPFHEVFNATRFASVYAWNRKRISNCVADYSVLYNFAPFFAFKCYGVSPTKLNDLCFTNVYADSFVIRGNEVSQIAPGQTGNIADYNYKLPDDFTGCVIAWNSNKLDSKVSGNYNYLYRLFRKSKLKPFERDISTEIYQAGNKPCNGVAGFNCYFPLQSYGFRPTYGVGHQPYRVVVLSFELLHAPATVCGPKKSTNLVKNK
Expression System	HEK293
Molecular Weight	Theoretical:26.5kDa Actual:35kDa
Purity	＞95% by SDS-PAGE
Endotoxin	<1EU/μg
Tag	His Tag
Physical Appearance	Lyophilized Powder
Storage Buffer	Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4.
Reconstitution	Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.
Stability & Storage	12 months from date of receipt, -20 to -70 °C as supplied. 6 months, -20 to -70 °C under sterile conditions after reconstitution. 1 week, 2 to 8 °C under sterile conditions after reconstitution. Please avoid repeated freeze-thaw cycles.

Background

Sars-cov-2 (2019-NCOV) infects human respiratory epithelial cells through binding to human ACE2 receptors. Spike protein is a large type I transmembrane protein containing two subunits S1 and S2.S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing cell surface receptors. S2 contains the basic elements required for membrane fusion. S protein plays a key role in inducing neutralizing antibody and T cell responses as well as protective immunity.

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Bioactivity

Immobilized SARS-CoV-2 Omicron RBD (BA.2.75), His tag at 4 μg/mL (50 μL/well) can bind Human ACE2/ACEH Protein with EC50 of 28.35-38.39 ng/mL.

SDS-PAGE

2μg（R: reducing conditions）

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SARS-CoV-2 Omicron RBD (BA.2.75), His tag