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SARS-CoV-2 Nucleocapsid protein , His tag

SARS-CoV-2 Nucleocapsid protein , His tag

Catalog Number: S0A2024 Reactivity: Viral Conjugation: Unconjugated Brand: Starter
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$131.00 SGD
$131.00 SGD
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Product Details

Product Specification


Species SARS-CoV-2
Accession P0DTC9
Research Area SARS-CoV-2
Amino Acid Sequence

Protein sequence(P0DTC9, Met1-Ala419, with C-10*His)
MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQAGGGGSHHHHHHHHHH
Expression System E.coli
Molecular Weight Theoretical: 47.3kDa Actual: 50kDa
Purity

>95% by SDS-PAGE
Endotoxin <1EU/μg
Conjugation Unconjugated
Physical Appearance Lyophilized Powder
Storage Buffer Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, 1mM EDTA, 1mM DTT, pH7.4. Normally trehalose is added as protectant before lyophilization.
Reconstitution Reconstitute no more than 1mg/mL according to the size in deionized water after rapid centrifugation.
Stability & Storage

· 12 months from date of receipt, -20 to -70 °C as supplied. 
· 6 months, -20 to -70 °C under sterile conditions after reconstitution.
· 1 week, 2 to 8 °C under sterile conditions after reconstitution.  
· Please avoid repeated freeze-thaw cycles.

Background

Severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2)[2] is a strain of coronavirus that causes COVID-19 (coronavirus disease 2019), the respiratory illness responsible for the ongoing COVID-19 pandemic.The nucleocapsid (N) protein is a protein that packages the coronaviruses to form ribonucleoprotein structures enclosed within the viral capsid. In addition to its interactions with RNA, N forms protein-protein interactions with the coronavirus membrane protein (M) during the process of viral assembly. N also has additional functions in manipulating the cell cycle of the host cell. The N protein is highly immunogenic and antibodies to N are found in patients recovered from SARS and Covid-19.

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Bioactivity

Immobilized SARS-CoV-2 Nucleocapsid protein, His tag at 4 μg/mL (50 μL/well) can bind SARS-CoV-2 Nucleocapsid Protein Recombinant Rabbit mAb (SDT-021-30) (S0B3009) with EC50 of 4.409-5.138 ng/ml.

SDS-PAGE

2μg (R: reducing conditions)