Immobilized SARS-CoV-2 Nucleocapsid Protein , His tag at 1 μg/mL (50 μL/well) can bind SARS-CoV-2 Nucleocapsid Protein Recombinant Rabbit mAb (SDT-021-30) (S0B3009) with EC50 of 5.018-6.825 ng/mL.
SARS-CoV-2 Nucleocapsid Protein , His tag
SARS-CoV-2 Nucleocapsid Protein , His tag
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Product Details
Product Details
Product Specification
| Species | SARS-CoV-2 |
| Synonyms | n/a |
| Accession | P0DTC9 |
| Amino Acid Sequence | Protein sequence(P0DTC9, Met1-Ala419, with C-10*His) MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQAGGGGSHHHHHHHHHH |
| Expression System | HEK293 |
| Molecular Weight | Theoretical:47.3kDa Actual:60kDa |
| Purity | ＞90% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4. |
| Reconstitution | Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation. |
| Stability & Storage | 12 months from date of receipt, -20 to -70 °C as supplied. 6 months, -20 to -70 °C under sterile conditions after reconstitution. 1 week, 2 to 8 °C under sterile conditions after reconstitution. Please avoid repeated freeze-thaw cycles. |
Background
Severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2)[2] is a strain of coronavirus that causes COVID-19 (coronavirus disease 2019), the respiratory illness responsible for the ongoing COVID-19 pandemic.The nucleocapsid (N) protein is a protein that packages the coronaviruses to form ribonucleoprotein structures enclosed within the viral capsid. In addition to its interactions with RNA, N forms protein-protein interactions with the coronavirus membrane protein (M) during the process of viral assembly. N also has additional functions in manipulating the cell cycle of the host cell. The N protein is highly immunogenic and antibodies to N are found in patients recovered from SARS and Covid-19.
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Bioactivity
SDS-PAGE
2μg (R: reducing conditions)
