WB result of SAMHD1 Recombinant Rabbit mAb
Primary antibody: SAMHD1 Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: HCC827 whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: Daudi whole cell lysate 20 µg
Lane 4: Ramos whole cell lysate 20 µg
Negative control: HCC827 whole cell lysate; Jurkat whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 73 kDa
Observed MW: 70 kDa
SAMHD1 Recombinant Rabbit mAb (S-2401-25)
SAMHD1 Recombinant Rabbit mAb (S-2401-25)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SAMHD1 |
| Synonyms | Deoxynucleoside triphosphate triphosphohydrolase SAMHD1; dNTPase; Dendritic cell-derived IFNG-induced protein (DCIP); Monocyte protein 5 (MOP-5); SAM domain and HD domain-containing protein 1 (hSAMHD1); MOP5 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | Q9Y3Z3 |
| Clone Number | S-2401-25 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | Daudi, Ramos |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:5000 | Hu |
| IHC-P | 1:100 | Hu |
Background
SAMHD1 (Sterile Alpha Motif and HD Domain-Containing Protein 1) is a protein that plays a crucial role in cellular processes. It is involved in the regulation of dNTP (deoxyribonucleoside triphosphate) levels within cells, which is essential for DNA synthesis and repair. SAMHD1 acts as a dNTP triphosphohydrolase, helping to maintain a balanced pool of dNTPs to prevent excessive DNA replication and genomic instability. Additionally, it has been implicated in innate immune responses, particularly in restricting the replication of certain viruses, such as HIV-1, by limiting the availability of dNTPs required for viral reverse transcription. Its dysfunction has been associated with various diseases, including Aicardi-Goutières syndrome, an autoimmune disorder characterized by chronic inflammation and neurological damage.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human spleen. Anti-SAMHD1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-SAMHD1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-SAMHD1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-SAMHD1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
