WB result of S100A9 Recombinant Rabbit mAb
Primary antibody: S100A9 Recombinant Rabbit mAb at 1/2000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: THP-1 whole cell lysate 20 µg
Lane 3: HL-60 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 14 kDa
Observed MW: 14 kDa
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | S100A9 |
| Synonyms | Protein S100-A9; Calgranulin-B; Calprotectin L1H subunit; Leukocyte L1 complex heavy chain; Migration inhibitory factor-related protein 14 (MRP-14; p14); S100 calcium-binding protein A9; CAGB; CFAG; MRP14 |
| Location | Cytoplasm, Cytoskeleton, Secreted, Cell membrane |
| Accession | P06702 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Positive Sample | MCF7, THP-1, HL-60 |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu |
Picture
Picture
Western Blot
