IHC shows positive staining in paraffin-embedded human testis. Anti-Steroidogenic Factor 1/SF-1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
S-RMab®Steroidogenic Factor 1/SF-1 Recombinant Rabbit mAb (SDT-1666-11)
S-RMab®Steroidogenic Factor 1/SF-1 Recombinant Rabbit mAb (SDT-1666-11)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Steroidogenic Factor 1/SF-1 |
| Synonyms | STF-1; hSF-1; Adrenal 4-binding protein; Fushi tarazu factor homolog 1; Nuclear receptor subfamily 5 group A member 1; Steroid hormone receptor Ad4BP; NR5A1; AD4BP, FTZF1, SF1 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | Q13285 |
| Clone Number | SDT-1666-11 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P |
| Reactivity | Hu |
| Positive Sample | human adrenal gland, human testis |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:100 | Hu |
Background
SF-1 (Steroidogenic Factor 1) is a 52-kDa nuclear receptor transcription factor that activates the promoters of many key genes involved in steroidogenesis, adrenal development, and reproduction (CYP11A, CYP11B, CYP21B, AHCH, STAR, etc.) within the body. It is expressed in the adrenal gland and reproductive axis both during fetal development and in adulthood. High expression is observed in the adrenal cortex, pituitary, ovaries (granulosa and theca cells), testes (sertoli and leydig cells), and spleen.
Picture
Picture
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human adrenal gland. Anti-Steroidogenic Factor 1/SF-1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian steroid cell tumor. Anti-Steroidogenic Factor 1/SF-1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
