S-RMabMixTM Tri-Methyl Lysine Rabbit mAb

Catalog Number: S0B6173 Application: ELISA, WB Reactivity: Species Independent Conjugation: Unconjugated Brand: Starter

Price:

$108 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	Tri-Methyl Lysine
Synonyms	Kme3, trimethylation
Immunogen	Synthetic Peptide
Antibody Type	Recombinant mAb Mix
Isotype	IgG
Application	ELISA, WB
Reactivity	Species Independent
Positive Sample	HeLa, mouse liver
Purification	Protein A
Concentration	5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS pH7.4
Stability & Storage	12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution

application	dilution	species
Dot Blot	1:5000
WB	1:1000	Hu, Ms

Background

Tri-Methyl Lysine (Kme3) is a post-translational modification (PTM) where three methyl groups (–CH₃) are added to the ε-amino group of a lysine residue, forming Nε-trimethyllysine. This modification is commonly found on histone proteins (e.g., H3K4me3, H3K9me3, H3K27me3) and plays a crucial role in regulating chromatin structure and gene expression by recruiting effector proteins such as chromatin remodelers and transcription factors. Trimethylation is catalyzed by lysine methyltransferases (KMTs, e.g., EZH2 for H3K27me3) and can be reversed by lysine demethylases (KDMs, e.g., JMJD2 family). Unlike mono- or di-methylation, trimethylation often marks stable epigenetic silencing (e.g., H3K27me3 in Polycomb repression) or active transcription (e.g., H3K4me3 at promoters). It is detected using antibody-based methods (ChIP-seq, IF, WB) or mass spectrometry, and its dysregulation is linked to cancer and developmental disorders.

Picture

Western Blot

WB result of S-RMabMix™ Tri-Methyl Lysine Rabbit mAb
Primary antibody: S-RMabMix™ Tri-Methyl Lysine Rabbit mAb at 1/100 dilution
Lane 1: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple

WB result of S-RMabMix™ Tri-Methyl Lysine Rabbit mAb
Primary antibody: S-RMabMix™ Tri-Methyl Lysine Rabbit mAb at 1/100 dilution
Lane 1: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple

Dot Blot

Dot blot result of S-RMabMix™ Tri-Methyl Lysine Rabbit mAb
Lane 1: Modified peptide library (Monomethyl Lysine)
Lane 2: Modified peptide library (Dimethyl Lysine)
Lane 3: Modified peptide library (Trimethyl Lysine)
Lane 4: Unmodified lysine peptide library control
Lane 5: Modified peptide library (Symmetric dimethyl Arginine)
Lane 6: Modified peptide library (Asymmetric dimethyl Arginine)
Lane 7: Modified peptide library (Monomethyl Arginine)
Lane 8: Unmodified arginine peptide library control
Primary antibody: S-RMabMix™ Tri-Methyl Lysine Rabbit mAb at 1/5000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution