WB result of Caspase-7 Recombinant Rabbit mAb
Primary antibody: Caspase-7 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated Jurkat whole cell lysate 20 µg
Lane 2: Jurkat treated with 1 µM staurosporine for 4 hours whole cell lysate 20 µg
Lane 3: untreated HeLa whole cell lysate 20 µg
Lane 4: HeLa treated with 1 µM staurosporine for 3 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 15, 28, 35 kDa
S-RMab® Caspase-7 Recombinant Rabbit mAb (S-1539-42)
S-RMab® Caspase-7 Recombinant Rabbit mAb (S-1539-42)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Caspase-7 |
| Synonyms | CASP-7; Apoptotic protease Mch-3; CMH-1; ICE-like apoptotic protease 3 (ICE-LAP3); MCH3; CASP7 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus, Secreted |
| Accession | P55210 |
| Clone Number | S-1539-42 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, Jurkat |
| Predicted Reactivity | Hm |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:500 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
Caspase-7 is a cysteine protease that plays a crucial role in the execution phase of apoptosis, the programmed cell death process essential for maintaining tissue homeostasis and development. Along with caspase-3 and caspase-6, it is classified as an executioner caspase, which is activated by initiator caspases such as caspase-8 and caspase-9. Initially thought to be functionally redundant with caspase-3, recent research has highlighted distinct roles for caspase-7. It has unique substrate preferences and is involved in specific cellular processes, including the repair of plasma membrane pores through the activation of acid sphingomyelinase (ASM) under inflammatory conditions. Additionally, caspase-7 is implicated in the regulation of inflammatory responses, particularly through its interaction with inflammasomes. Its distinct functions and involvement in both apoptosis and inflammation make caspase-7 a potential therapeutic target for diseases related to these processes, such as cancer and inflammatory disorders.
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Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti- Caspase-7 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti- Caspase-7 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti- Caspase-7 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in Jurkat cells. Anti- Caspase-7 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
