WB result of Nectin-4 rabbit mAb
Primary antibody: Nectin-4 rabbit mAb at 1/1000 dilution
Lane 1: A549 whole cell lysate 20 µg
Lane 2: T47D whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Negative control: A549 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 38~70 kDa
S-RMab® Nectin-4 Recombinant Rabbit mAb (SDT-277-314)
S-RMab® Nectin-4 Recombinant Rabbit mAb (SDT-277-314)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | NECTIN-4 |
| Synonyms | LNIR, PRR4, PVRL4 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | Q96NY8 |
| Clone Number | SDT-277-314 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | |
| IHC-P | 1:500 |
Background
Nectin-4 (Nectin cell adhesion molecule 4) is a type I transmembrane cell adhesion molecule belonging to the Nectin family. A homolog formerly known as the poliovirus receptor (PVR/CD155) is also known as the poliovirus receptor associated (PRR) protein. During physiological development, Nectin-4 is specifically expressed during embryonic and fetal development and is very low expressed in adult tissues. It forms physical connections between neighboring cells and is essential for enabling intercellular communication, migration, and other important cellular processes. Nectin-4 is overexpressed in a variety of tumor cells, and Nectin-4 is used as a marker for cancer recurrence and metastasis, which is associated with poor prognosis of a variety of cancers, including uroepithelial carcinoma, breast cancer, ovarian cancer, pancreatic cancer, non-small cell lung cancer, gastric cancer, hepatocellular carcinoma and bladder cancer. Nectin-4 can promote tumor cell proliferation and differentiation, angiogenesis, lymphangiogenesis and lymphatic metastasis through activation of PI3K/AKT pathway, playing an important role in the occurrence and metastasis of cancer. Nectin-4 is also an independent biomarker associated with poor overall survival in some cancer types. Due to its high specific expression in tumors, drug studies targeting this target have emerged.
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Western Blot
WB result of Nectin-4 rabbit mAb
Primary antibody: Nectin-4 rabbit mAb at 1/2000 dilution
Lane 1: Nectin-4 His Tag, Human (recombinant protein) lysate 5 ng
Lane 2: Nectin-1 His Tag, Human (recombinant protein) lysate 5 ng
Lane 3: Nectin-2 His Tag, Human (recombinant protein) lysate 5 ng
Lane 4: Nectin-3 His Tag, Human (recombinant protein) lysate 5 ng
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44~47 kDa
Observed MW: 44 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Nectin-4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-Nectin-4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Nectin-4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Nectin-4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
