| Host | Rabbit |
| Antigen | Cytokeratin 7 |
| Synonyms | Cytokeratin-7 (CK-7), Keratin-7 (K7), Sarcolectin, Type-II keratin Kb7, KRT7 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P08729 |
| Clone Number | SDT-121-17 |
| Antibody Type | Rabbit mAb |
| Application | WB, IHC-P, ICC, FCM, IP, IF |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5mg/ml |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
| application | dilution | species |
| IP | 1:25 | |
| ICC | 1:500 | |
| IHC-P | 1:500 | |
| FCM | 1:500 | |
| WB | 1:1000 | |
| IF | 1:200 |
Cytokeratin 7 is the intermediate silk protein mainly expressed in epithelial cells. The heterodine of keratin consists of a polymerization filament by an acid -keratin (or type I keratin, keratin 9 to 23) and alkaline keratin (or type II keratin, keratin 1 to 8). Expression similar to but more limited than keratins 8 and 18 in simple, pseudostratified and ductal epithelium, mesothelium and urothelium.It's enerally expressed (with some variation) in adenocarcinoma of lung, breast, thyroid, endometrium, cervix, ovary, salivary gland, upper GI tract, urothelial carcinoma, papillary renal cell carcinoma and Paget disease It's generally negative (with some variation) in colorectal carcinoma, Merkel cell carcinoma, hepatocellular carcinoma, prostatic adenocarcinoma, adrenocortical tumors and squamous cell carcinoma.
WB result of Cytokeratin 7 Rabbit mAb
Primary antibody: Cytokeratin 7 Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: Hela whole cell lysate 20 µg
Lane 4: SK-BR-3 whole cell lysate 20 µg
Negative control: MCF7 whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40~55 kDa
Observed MW: 40~55 kDa
Exposure time: 40s
Flow cytometric analysis of MCF7 (Human monocytic leukemia monocyte, left) / HeLa (Human T cell leukemia T lymphocyte, right) cells labelling Cytokeratin 7 antibody at 1/500 dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG Alexa Fluor® 488 at 1/1000 dilution was used as the secondary antibody. Negative control: MCF7
Cytokeratin 7 Rabbit mAb at 1/25 dilution (2µg) immunoprecipitating Cytokeratin 7 in 0.4mg SK-BR-3 whole cell lysate. Western blot was performed on the immunoprecipitate using Cytokeratin 7 Rabbit mAb at 1/1000 dilution. Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: SK-BR-3 whole cell lysate 10µg (input)
Lane 2: Cytokeratin 7 Rabbit mAb IP in SK-BR-3 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in SK-BR-3 whole cell lysate
Predicted MW: 40~55 kDa
Observed MW: 40~55 kDa
Exposure time: 0.5s
IHC shows positive staining in paraffin-embedded human breast cancer.
Anti-Cytokeratin 7 antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast.
Anti-Cytokeratin 7 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma cancer.
Anti-Cytokeratin 7 antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer.
Anti-Cytokeratin 7 antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human bladder cancer.
Anti-Cytokeratin 7 antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta.
Anti-Cytokeratin 7 antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control.IHC shows negative staining in paraffin-embedded human colon.Anti-Cytokeratin 7 antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ICC shows positive staining in HeLa cells (top panel) and negative staining in MCF7 cells (below panel). Anti-Cytokeratin 7 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
IF shows positive staining in paraffin-embedded human breast cancer. Anti-Cytokeratin 7 antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-Cytokeratin 7 antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
Negative control: IF shows negative staining in paraffin-embedded human gastrointestinal stromal tumor. Anti-EpCAM antibody was used at 1/200 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
