WB result of RSK1/RSK2/RSK3 Recombinant Rabbit mAb
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: RSK1/RSK2/RSK3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Lane 5: HT-29 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 83 kDa
Observed MW: 85 kDa
RSK1/RSK2/RSK3 Recombinant Rabbit mAb (S-4982)
RSK1/RSK2/RSK3 Recombinant Rabbit mAb (S-4982)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | RSK1/RSK2/RSK3 |
| Synonyms | Ribosomal protein S6 kinase alpha-1; S6K-alpha-1; 90 kDa ribosomal protein S6 kinase 1 (p90-RSK 1; p90RSK1; p90S6K); MAP kinase-activated protein kinase 1a (MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a); Ribosomal S6 kinase 1 (RSK-1); RPS6KA1; MAPKAPK1A; RSK1; Ribosomal protein S6 kinase alpha-2; S6K-alpha-2; 90 kDa ribosomal protein S6 kinase 2 (p90-RSK 2; p90RSK2); MAP kinase-activated protein kinase 1c (MAPK-activated protein kinase 1c; MAPKAP kinase 1c; MAPKAPK-1c); Ribosomal S6 kinase 3 (RSK-3); pp90RSK3; RPS6KA2; MAPKAPK1C; RSK3; Ribosomal protein S6 kinase alpha-3; S6K-alpha-3; 90 kDa ribosomal protein S6 kinase 3 (p90-RSK 3; p90RSK3); Insulin-stimulated protein kinase 1 (ISPK-1); MAP kinase-activated protein kinase 1b (MAPK-activated protein kinase 1b; MAPKAP kinase 1b; MAPKAPK-1b); Ribosomal S6 kinase 2 (RSK-2); pp90RSK2; RPS6KA3; ISPK1; MAPKAPK1B; RSK2 |
| Location | Cytoplasm, Nucleus |
| Accession | Q15418、P51812、Q15349 |
| Clone Number | S-4982 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HeLa, K562, A431, MCF7, HT-29, L-929, NIH/3T3, RAW264.7, AR42J, PC-12, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt, Mk |
Background
Ribosomal S6 Kinases 1, 2, and 3 (RSK1, RSK2, and RSK3) are serine/threonine kinases belonging to the p90 ribosomal S6 kinase (p90RSK) family, which function as critical downstream effectors of the Ras-MAPK/ERK signaling pathway to regulate diverse cellular processes including proliferation, survival, differentiation, and metabolism. These isoforms share a unique bimodal structure comprising an N-terminal kinase domain and a C-terminal kinase domain linked by a regulatory loop, where activation typically involves phosphorylation by ERK1/2 at the C-terminal domain followed by autophosphorylation and subsequent activation of the N-terminal domain to phosphorylate various substrates such as CREB, Bad, and GSK3β. While they exhibit high sequence homology and overlapping functions, RSK1, RSK2, and RSK3 display distinct tissue distribution patterns and specific physiological roles; for instance, RSK2 mutations are directly linked to Coffin-Lowry syndrome, a rare X-linked genetic disorder characterized by severe psychomotor retardation and skeletal abnormalities, highlighting the non-redundant importance of specific RSK isoforms in neural development and cognitive function, whereas RSK1 and RSK3 are often implicated in cancer progression and cardiac hypertrophy, making them significant targets for therapeutic intervention in oncology and cardiovascular diseases.
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Western Blot
WB result of RSK1/RSK2/RSK3 Recombinant Rabbit mAb
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: RSK1/RSK2/RSK3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: L-929 whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Lane 3: RAW264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 83 kDa
Observed MW: 85 kDa
WB result of RSK1/RSK2/RSK3 Recombinant Rabbit mAb
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: RSK1/RSK2/RSK3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: AR42J whole cell lysate 20 µg
Lane 2: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 83 kDa
Observed MW: 85 kDa
WB result of RSK1/RSK2/RSK3 Recombinant Rabbit mAb
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: RSK1/RSK2/RSK3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 83 kDa
Observed MW: 85 kDa
