WB result of RPS3A Rabbit pAb
Primary antibody: RPS3A Rabbit pAb at 1/2000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: 293T whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 30 kDa
RPS3A Rabbit polyclonal antibody
RPS3A Rabbit polyclonal antibody
Price:
Regular price
$131.00 SGD
Regular price
Sale price
$131.00 SGD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | Small ribosomal subunit protein eS1, 40S ribosomal protein S3a, v-fos transformation effector protein (Fte-1), FTE1, MFTL |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P61247 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P, IP |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Av, Rb, Pr, Sn, Mq, Ct |
| Purification | Immunogen Affinity |
| Concentration | 0.1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:2000 | |
| IHC-P | 1:100 | |
| IP | 1:25 |
Background
RPS3A, also known as 40S ribosomal protein S3a, is a protein that in humans is encoded by the RPS3A gene. It plays a role in ribosomal structure formation and protein binding, participating in protein biosynthesis. Recently, researchers found that the NAL21 gene in rice, which encodes RPS3A, regulates leaf width by modulating the translation levels of growth factor and leaf lateral expansion regulators. This study suggests that appropriate translation regulation of key transcription factors during leaf development is essential for maintaining normal leaf morphology. Moreover, RPS3A has been associated with certain diseases, such as spherocytosis types 1 and 4. It is also involved in pathways related to viral mRNA translation and metabolism.
Picture
Picture
Western Blot
WB result of RPS3A Rabbit pAb
Primary antibody: RPS3A Rabbit pAb at 1/2000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 30 kDa
WB result of RPS3A Rabbit pAb
Primary antibody: RPS3A Rabbit pAb at 1/2000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 35 kDa
IP
RPS3A Rabbit pAb at 1/25 dilution (0.4 µg) immunoprecipitating RPS3A in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using RPS3A Rabbit pAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 20 µg (Input)
Lane 2: RPS3A Rabbit pAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 30 kDa
Observed MW: 30 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti- RPS3A antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti- RPS3A antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti- RPS3A antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti- RPS3A antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
