RPS3 Recombinant Rabbit mAb (S-4121-27)

Catalog Number: S0B6977 Application: WB, IHC-P, ICC Reactivity: Hu, Ms, Rt, Mk Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

Quantity:

For shipping services or bulk orders, you may request a quotation.

Secure checkout with

View full details

Product Details

Product Specification

Host	Rabbit
Antigen	RPS3
Synonyms	Small ribosomal subunit protein uS3
Immunogen	Synthetic Peptide
Location	Cytoplasm, Cytoskeleton, Nucleus, Mitochondrion
Accession	P23396
Clone Number	S-4121-27
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P, ICC
Reactivity	Hu, Ms, Rt, Mk
Positive Sample	HeLa, K562, HepG2, Jurkat, NIH/3T3, Neuro-2a, PC-12, COS-7
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms, Rt, Mk
IHC-P	1:1000	Hu, Ms, Rt
ICC	1:500	Hu

Background

Ribosomal Protein S3 (RPS3) is a multifunctional component of the 40S small ribosomal subunit in eukaryotes, primarily recognized for its essential role in protein synthesis by facilitating the accurate decoding of mRNA and maintaining translational fidelity through its interaction with the 18S rRNA. Beyond its canonical function in translation, RPS3 exhibits significant "moonlighting" activities, particularly as an extraribosomal protein involved in DNA repair mechanisms, where it acts as a DNA glycosylase to remove oxidized bases such as 8-oxoguanine, thereby protecting genomic integrity against oxidative stress. Furthermore, RPS3 participates in various cellular signaling pathways, including the regulation of apoptosis and the NF-κB pathway, and has been implicated in viral infection processes, such as serving as a receptor or cofactor for certain viruses, highlighting its critical importance in both fundamental cellular homeostasis and disease pathogenesis.

Picture

Western Blot

WB result of RPS3 Recombinant Rabbit mAb
Primary antibody: RPS3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 30 kDa

WB result of RPS3 Recombinant Rabbit mAb
Primary antibody: RPS3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: Neuro-2a whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 30 kDa

WB result of RPS3 Recombinant Rabbit mAb
Primary antibody: RPS3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 30 kDa

WB result of RPS3 Recombinant Rabbit mAb
Primary antibody: RPS3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 30 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human brain. Anti-RPS3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-RPS3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse brain. Anti-RPS3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat kidney. Anti-RPS3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunocytochemistry

ICC shows positive staining in K562 cells. Anti- RPS3 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).