RPLP0 Rabbit Polyclonal Antibody

Catalog Number: S0B60544 Application: WB, IHC-P Reactivity: Hu, Ms, Rt, Mk Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	RPLP0
Immunogen	Synthetic Peptide
Location	Cytoplasm, Nucleus
Accession	P05388
Antibody Type	Polyclonal antibody
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt, Mk
Positive Sample	HeLa, Jurkat, Ramos, RAW264.7, mouse spleen, C6, rat spleen, COS-7
Predicted Reactivity	Bv, Rb, Pg, Ck, Zf, Dr
Purification	Immunogen Affinity
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms, Rt
IHC-P	1:50	Hu, Ms, Rt

Background

RPLP0 (ribosomal protein lateral stalk subunit P0, also known as uL10) is the core structural protein of the P-stalk of the 60S large ribosomal subunit, encoded by the RPLP0 gene located on chromosome 12q24.23. As the functional equivalent of Escherichia coli L10 protein, RPLP0 belongs to the L10P family, and its C-terminus is highly similar to the acidic ribosomal phosphoproteins P1 and P2. By binding to P1/P2 dimers, it forms a pentameric complex composed of a P1₂-P2₂ tetramer and a P0 monomer; this structure is essential for GTP hydrolysis during translation and for the recruitment of translation factors such as eEF2. RPLP0 is widely expressed in normal tissues, but recent studies have revealed that it is significantly upregulated in various tumors including clear cell renal cell carcinoma and lung adenocarcinoma, where high expression is independently associated with advanced stage, metastasis, and poor prognosis; its mechanisms involve promoting epithelial-mesenchymal transition (EMT), regulating the cell cycle, and influencing the immune microenvironment. Notably, RPLP0 also has a nuclear function independent of the ribosome: DNA damage-induced paraspeckle formation can recruit RPLP0 to bind the NONO protein, enhancing DNA-PK autophosphorylation and thereby promoting non-homologous end joining repair and tumor radioresistance. In addition, owing to its stable expression, RPLP0 is often used as a reference gene for RT-qPCR and has been validated as one of the most stable reference genes in studies such as osteogenic differentiation of stem cells.

Picture

Western Blot

WB result of RPLP0 Rabbit Polyclonal Antibody
Primary antibody: RPLP0 Rabbit Polyclonal Antibody at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: Ramos whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 36 kDa

WB result of RPLP0 Rabbit Polyclonal Antibody
Primary antibody: RPLP0 Rabbit Polyclonal Antibody at 1/1000 dilution
Lane 1: RAW 264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 36 kDa

WB result of RPLP0 Rabbit Polyclonal Antibody
Primary antibody: RPLP0 Rabbit Polyclonal Antibody at 1/1000 dilution
Lane 1: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 36 kDa
This blot was developed with high sensitivity substrate

WB result of RPLP0 Rabbit Polyclonal Antibody
Primary antibody: RPLP0 Rabbit Polyclonal Antibody at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 36 kDa

WB result of RPLP0 Rabbit Polyclonal Antibody
Primary antibody: RPLP0 Rabbit Polyclonal Antibody at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 36 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human liver. Anti-RPLP0 antibody was used at 1/50 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon. Anti-RPLP0 antibody was used at 1/50 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-RPLP0 antibody was used at 1/50 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-RPLP0 antibody was used at 1/50 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse liver. Anti-RPLP0 antibody was used at 1/50 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat liver. Anti-RPLP0 antibody was used at 1/50 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat brain. Anti-RPLP0 antibody was used at 1/50 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.