WB result of RPA32/RPA2 Rabbit pAb
Primary antibody: RPA32/RPA2 Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: HUVEC whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 29 kDa
Observed MW: 29 kDa
RPA32/RPA2 Rabbit Polyclonal Antibody
RPA32/RPA2 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | RPA32 |
| Synonyms | Replication protein A 32 kDa subunit; RP-A p32; Replication factor A protein 2 (RF-A protein 2); Replication protein A 34 kDa subunit (RP-A p34); REPA2; RPA34 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P15927 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, Jurkat, HUVEC, NIH/3T3, C6 |
| Predicted Reactivity | Or |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
Background
RPA32, also known as the 32 kDa subunit of Replication Protein A (RPA), is an essential component of the RPA complex. RPA is a highly conserved single-stranded DNA (ssDNA) binding protein in eukaryotes, composed of three subunits: RPA70, RPA32, and RPA14. RPA32 binds to ssDNA during DNA replication and repair, and plays a crucial role in DNA damage response. Particularly, the phosphorylation of threonine 21 (Thr21) in RPA32 is vital for effective DNA repair, especially in homologous recombination (HR), as it enhances RPA's binding affinity to ssDNA and promotes the recruitment of Rad51, a key player in HR that contributes to genomic stability.
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Western Blot
WB result of RPA32/RPA2 Rabbit pAb
Primary antibody: RPA32/RPA2 Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 29 kDa
Observed MW: 29 kDa
WB result of RPA32/RPA2 Rabbit pAb
Primary antibody: RPA32/RPA2 Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 29 kDa
Observed MW: 29 kDa
