RPA3 Recombinant Rabbit mAb (S-4552-20)

Catalog Number: S0B60334 Application: WB, IHC-P Reactivity: Hu Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl 1ml

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Product Details

Product Specification

Host	Rabbit
Antigen	RPA3
Synonyms	Replication protein A 14 kDa subunit; REPA3; RPA14
Immunogen	Synthetic Peptide
Location	Nucleus
Accession	P35244
Clone Number	S-4552-20
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu
Positive Sample	SK-OV-3, HepG2, HeLa, MCF7, Ramos
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:2000	Hu
IHC-P	1:200	Hu

Background

RPA3, also known as Replication Protein A3, is the smallest subunit (14 kDa) of the heterotrimeric replication protein A (RPA) complex, encoded by a gene located on human chromosome 7p21.3. It comprises 121 amino acids and features an oligonucleotide/oligosaccharide-binding (OB-fold) domain as its structural core. As the structural scaffold of the RPA complex, RPA3 is essential for the assembly and stability of the entire complex. Through tight association with the RPA1 (70 kDa) and RPA2 (32 kDa) subunits, it forms a single-stranded DNA (ssDNA)-binding platform that plays a central role in nearly all aspects of DNA metabolism, including DNA replication, recombination, and repair. Upon cellular detection of DNA damage, the RPA complex rapidly binds to and stabilizes exposed ssDNA intermediates. This process not only prevents re-annealing of DNA but also activates the ATR-dependent DNA damage checkpoint by recruiting key factors such as the ATRIP protein kinase, and further recruits downstream repair proteins including RAD51 and XPA to damage sites, initiating pathways such as homologous recombination repair and nucleotide excision repair. Additionally, RPA3 can form an alternative RPA complex (aRPA) with RPA4 (30 kDa), potentially functioning in certain specific DNA repair contexts. Aberrations in RPA3 itself or its interacting factors—such as RFWD3-mediated ubiquitination—have also been found to be genetically associated with various diseases including lung cancer, head and neck cancer, and myocardial infarction.

Picture

Western Blot

WB result of RPA3 Recombinant Rabbit mAb
Primary antibody: RPA3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: SK-OV-3 whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Lane 5: Ramos whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 13 kDa
Observed MW: 13 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human spleen. Anti-RPA3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human testis. Anti-RPA3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human tonsil. Anti-RPA3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver cancer. Anti-RPA3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-RPA3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.