WB result of ROBO1 Recombinant Rabbit mAb
Primary antibody: ROBO1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: LoVo whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: PANC-1 whole cell lysate 20 µg
Lane 5: HeLa whole cell lysate 20 µg
Lane 6: BxPC-3 whole cell lysate 20 µg
Lane 7: A549 whole cell lysate 20 µg
Lane 8: U-2 OS whole cell lysate 20 µg
Negative control: LoVo whole cell lysate
Low expression control: MCF7 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 181 kDa
Observed MW: 260 kDa
ROBO1 Recombinant Rabbit mAb (S-2931-169)
ROBO1 Recombinant Rabbit mAb (S-2931-169)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ROBO1 |
| Synonyms | Roundabout homolog 1; Deleted in U twenty twenty; H-Robo-1; DUTT1 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | Q9Y6N7 |
| Clone Number | S-2931-169 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HepG2, PANC-1, HeLa, BxPC-3, A549, U-2 OS, mouse E14 embryo, rat E14 embryo |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:500 | Hu |
| ICC | 1:500 | Hu |
Background
ROBO1 is a ~165 kDa single-pass transmembrane receptor of the immunoglobulin superfamily whose extracellular region contains five Ig-like domains and three fibronectin repeats that bind the secreted SLIT1/2 ligands, while its cytoplasmic tail carries four conserved CC motifs (CC0-CC3) that recruit adaptors such as srGAPs, Ena/VASP and DCC to transduce repulsive or attractive signals regulating axon guidance at the CNS midline, neuronal precursor migration, angiogenesis and even tumor metastasis by modulating Rho-family GTPases and actin cytoskeleton dynamics.
Picture
Picture
Western Blot
WB result of ROBO1 Recombinant Rabbit mAb
Primary antibody: ROBO1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse E14 embryo lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 181 kDa
Observed MW: 260 kDa
WB result of ROBO1 Recombinant Rabbit mAb
Primary antibody: ROBO1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat E14 embryo lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 181 kDa
Observed MW: 260 kDa
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-ROBO1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
