WB result of RNA polymerase II CTD repeat YSPTSPS (phospho S7) Rat mAb Primary antibody: RNA polymerase II CTD repeat YSPTSPS (phospho S7) Rat mAb at 1/4000 dilution Lane 1: HeLa whole cell lysate 20 µg Lane 2: 293T whole cell lysate 20 µg Lane 3: HeLa whole cell lysate (phosphatase treated) 20 µg Lane 4: 293T whole cell lysate (phosphatase treated) 20 µg Secondary antibody: Goat Anti-Rat IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 217 kDa Observed MW: 260 kDa
RNA polymerase II CTD repeat YSPTSPS (phospho S7) Recombinant Rat mAb (S-R198)
RNA polymerase II CTD repeat YSPTSPS (phospho S7) Recombinant Rat mAb (S-R198)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | RNA polymerase II CTD repeat YSPTSPS (phosphor S7) |
| Synonyms | DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, POLR2A |
| Location | Cytoplasm, Nucleus |
| Accession | P24928 |
| Clone Number | S-R198 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG1 |
| Application | WB, ICC, IP |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein G |
| Concentration | 0.4 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:4000 | null |
| ICC | 1:100 | null |
| IP | 1:40 | null |
Background
RNA polymerase II (RNAP II and Pol II) is a multiprotein complex that transcribes DNA into precursors of messenger RNA (mRNA) and most small nuclear RNA (snRNA) and microRNA. The purified enzyme has typically 10–12 subunits (12 in humans and yeast). The subunit RPB1 contains a carboxy terminal domain (CTD) composed of up to 52 heptapeptide repeats (YSPTSPS) that are essential for polymerase activity.
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Western Blot
WB result of RNA polymerase II CTD repeat YSPTSPS (phospho S7) Rat mAb Primary antibody: RNA polymerase II CTD repeat YSPTSPS (phospho S7) Rat mAb at 1/4000 dilution Lane 1: RAW 264.7 whole cell lysate 20 µg Lane 2: C2C12 whole cell lysate 20 µg Lane 3: RAW264.7 whole cell lysate (phosphatase treated) 20 µg Lane 4: C2C12 whole cell lysate (phosphatase treated) 20 µg Secondary antibody: Goat Anti-Rat IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 217 kDa Observed MW: 260 kDa
IP
RNA polymerase II CTD repeat YSPTSPS (phosphor S7) Rat mAb at 1/40 dilution (1 µg) immunoprecipitating RNA polymerase II CTD repeat YSPTSPS (phosphor S7) in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using RNA polymerase II CTD repeat YSPTSPS (phosphor S7) Rat mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/10000 dilution.
Lane 1: HeLa whole cell lysate 20 µg (Input)
Lane 2: RNA polymerase II CTD repeat YSPTSPS (phosphor S7) Rat mAb IP in HeLa whole cell lysate
Lane 3: Rat monoclonal IgG1 IP in HeLa whole cell lysate
Predicted MW: 217 kDa
Observed MW: 260 kDa
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S7) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rat IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI.
