WB result of RNA polymerase II CTD repeat YSPTSPS (phospho S5) Recombinant Mouse mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: RNA polymerase II CTD repeat YSPTSPS (phospho S5) Recombinant Mouse mAb
at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: HeLa treated with phosphatase whole cell lysate 20 µg
Lane 4: 293T treated with phosphatase whole cell lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 218 kDa
Observed MW: 280 kDa
RNA polymerase II CTD repeat YSPTSPS (phospho S5) Recombinant Mouse mAb (S-3420)
RNA polymerase II CTD repeat YSPTSPS (phospho S5) Recombinant Mouse mAb (S-3420)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | RNA polymerase II CTD repeat YSPTSPS (phospho S5) |
| Synonyms | DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; 3'-5' exoribonuclease; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA-directed RNA polymerase II subunit RPB1; POLR2; POLR2A |
| Location | Cytoplasm, Nucleus |
| Accession | P24928 |
| Clone Number | S-3420 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1 |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, 293T, NIH/3T3 |
| Purification | Protein G |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu, Ms |
Background
RNA polymerase II CTD repeat YSPTSPS (phospho S5) is a crucial component of the RNA polymerase II complex, specifically referring to the phosphorylated serine 5 residue within the heptapeptide repeat sequence YSPTSPS in the CTD (C-terminal domain) of the largest subunit of RNA polymerase II. This phosphorylation of serine 5 plays a significant role in the transcription process, particularly during the initiation and early elongation phases. It helps in the recruitment of transcription factors and other regulatory proteins, facilitating the assembly of the pre-initiation complex and enabling efficient transcription initiation. Additionally, the phosphorylation status of S5 can influence the interaction of RNA polymerase II with other components of the transcription machinery, thereby regulating gene expression and ensuring proper processing of nascent RNA transcripts.
Picture
Picture
Western Blot
WB result of RNA polymerase II CTD repeat YSPTSPS (phospho S5) Recombinant Mouse mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: RNA polymerase II CTD repeat YSPTSPS (phospho S5) Recombinant Mouse mAb
at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with phosphatase whole cell lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 218 kDa
Observed MW: 280 kDa
WB result of RNA polymerase II CTD repeat YSPTSPS (phospho S5) Recombinant Mouse mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: RNA polymerase II CTD repeat YSPTSPS (phospho S5) Recombinant Mouse mAb
at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: PC-12 treated with phosphatase whole cell lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 218 kDa
Observed MW: 280 kDa
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
