WB result of RNA polymerase II CTD repeat YSPTSPS (phospho S2) Rat mAb
Primary antibody: RNA polymerase II CTD repeat YSPTSPS (phospho S2) Rat mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate (phosphatase treated) 20 µg
Lane 4: 293T whole cell lysate (phosphatase treated) 20 µg
Secondary antibody: Goat Anti-Rat IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 217 kDa
Observed MW: 270 kDa
Exposure time: 90 s
RNA polymerase II CTD repeat YSPTSPS (phospho S2) Recombinant Rat mAb (S-R199)
RNA polymerase II CTD repeat YSPTSPS (phospho S2) Recombinant Rat mAb (S-R199)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | RNA polymerase II CTD repeat YSPTSPS (phospho S2) |
| Synonyms | DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA-directed RNA polymerase II subunit RPB1; POLR2A |
| Location | Cytoplasm, Nucleus |
| Accession | P24928 |
| Clone Number | S-R199 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG1 |
| Application | WB, IHC-P, ICC, IF |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein G |
| Concentration | 0.4 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IHC | 1:400 | |
| ICC | 1:100 | |
| IF | 1:100 |
Background
RNA polymerase II (RNAP II and Pol II) is a multiprotein complex that transcribes DNA into precursors of messenger RNA (mRNA) and most small nuclear RNA (snRNA) and microRNA. The purified enzyme has typically 10–12 subunits (12 in humans and yeast). The subunit RPB1 contains a carboxy terminal domain (CTD) composed of up to 52 heptapeptide repeats (YSPTSPS) that are essential for polymerase activity.
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Western Blot
WB result of RNA polymerase II CTD repeat YSPTSPS (phospho S2) Rat mAb
Primary antibody: RNA polymerase II CTD repeat YSPTSPS (phospho S2) Rat mAb at 1/1000 dilution
Lane 1: RAW 264.7 whole cell lysate 20 µg
Lane 2: C2C12 whole cell lysate 20 µg
Lane 3: RAW264.7 whole cell lysate (phosphatase treated) 20 µg
Lane 4: C2C12 whole cell lysate (phosphatase treated) 20 µg
Secondary antibody: Goat Anti-Rat IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 217 kDa
Observed MW: 270 kDa
Exposure time: 90 s
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody was used at 1/400 dilution, followed by a HRP Polymer for Goat Anti-Rat IgG (H+L). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human testis (phosphatase treated). Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody was used at 1/400 dilution, followed by a HRP Polymer for Goat Anti-Rat IgG (H+L). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody was used at 1/400 dilution, followed by a HRP Polymer for Goat Anti-Rat IgG (H+L). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse testis (phosphatase treated). Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody was used at 1/400 dilution, followed by a HRP Polymer for Goat Anti-Rat IgG (H+L). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody was used at 1/400 dilution, followed by a HRP Polymer for Goat Anti-Rat IgG (H+L). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody was used at 1/400 dilution, followed by a HRP Polymer for Goat Anti-Rat IgG (H+L). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat testis (phosphatase treated). Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody was used at 1/400 dilution, followed by a HRP Polymer for Goat Anti-Rat IgG (H+L). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody was used at 1/400 dilution, followed by a HRP Polymer for Goat Anti-Rat IgG (H+L). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells (Right, Green) and negative staining in phosphatase treated (37 °C, 2h) HeLa cells (Left). Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody was used at 1/100 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rat IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI.
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse testis. Anti- RNA polymerase II CTD repeat YSPTSPS (phosphor S2) antibody was used at 1/100 dilution (magenta) and incubated overnight at 4°C. Goat Anti-Rat IgG(H+L) (Alexa Fluor® 647 Conjugate) (S0B4049) was used as secondary antibody at 1/2000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat testis. Anti- RNA polymerase II CTD repeat YSPTSPS (phosphor S2) antibody was used at 1/100 dilution (magenta) and incubated overnight at 4°C. Goat Anti-Rat IgG(H+L) (Alexa Fluor® 647 Conjugate) (S0B4049) was used as secondary antibody at 1/2000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
