The RIPK1 activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the RIPK1 protein, ATP at 25℃ for 120 min, then adding ADP-Glo reagent and reading RLU signal with BMG.
RIPK1 Protein
RIPK1 Protein
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | RIP, RIP1 |
| Accession | Q13546-1 |
| Amino Acid Sequence |
M1-A327 N-terminal GST tag FHRTQGLMIMKTVYKGPNCEHNEALLEEAKMMNRLRHSRVVKLLGVIIEEGKYSLVMEYMEKGNLMHVLKAEMSTPLSVKGRIILEIIEGMCYLHGKGVIHKDLKPENILVDNDFHIKIADLGLASFKMWSKLNNEEHNELREVDGTAKKNGGTLYYMAPEHLNDVNAKPTEKSDVYSFAVVLWAIFANKEPYENAICEQQLIMCIKSGNRPDVDDITEYCPREIISLMKLCWEANPEARPTFPGIEEKFRPFYLSQLEESVEEDVKSLKKEYSNENAVVKRMQSLQLDCVA |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 63.6 kDa |
| Purity | >90% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | GST Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 50 mM Tris, 150 mM NaCl, 10 mM Reduced Glu, 1 mM DTT, 10% glycerol, pH 7.5 |
| Stability & Storage |
Stable for
12 months upon stored at -80℃ from the date of receipt. And avoid repeated
freeze-thaws cycles.
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Picture
Picture
Bioactivity
SDS-PAGE
M:MW 1:1μg RIPK1 2:3μg RIPK1
SEC-HPLC
