WB result of Rictor Recombinant Rabbit mAb
Primary antibody: Rictor Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: SH-SY5Y whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: PANC-1 whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 192 kDa
Observed MW: 200 kDa
Rictor Recombinant Rabbit mAb (S-2601-26)
Rictor Recombinant Rabbit mAb (S-2601-26)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Rictor |
| Synonyms | Rapamycin-insensitive companion of mTOR; AVO3 homolog (hAVO3); KIAA1999; RICTOR |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane, Endoplasmic reticulum membrane |
| Accession | Q6R327 |
| Clone Number | S-2601-26 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | SH-SY5Y, HeLa, PANC-1, HepG2, NIH/3T3, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Mk |
| IHC-P | 1:200 | Hu, Ms, Rt |
Background
Rictor (rapamycin-insensitive companion of mTOR) is a 1,709-amino-acid, 190 kDa cytosolic scaffold protein that is an obligate core subunit of mTORC2, where it stabilizes the complex and recruits substrates such as AKT, PKCα and SGK1 for phosphorylation by mTOR on key hydrophobic motifs, thereby controlling cell survival, metabolism, cytoskeletal organization, embryonic stem-cell differentiation and lymphatic valve formation, while its frequent overexpression or mutation in cancers links it to tumor growth, invasion and drug resistance.
Picture
Picture
Western Blot
WB result of Rictor Recombinant Rabbit mAb
Primary antibody: Rictor Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 192 kDa
Observed MW: 200 kDa
WB result of Rictor Recombinant Rabbit mAb
Primary antibody: Rictor Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 192 kDa
Observed MW: 200 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human liver. Anti-Rictor antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-Rictor antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-Rictor antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Rictor antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-Rictor antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-Rictor antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
