WB result of Rheb Rabbit pAb
Primary antibody: Rheb Rabbit pAb at 1/1000 dilution
Lane 1: A549 whole cell lysate 20 µg
Lane 2: U-87 MG whole cell lysate 20 µg
Lane 3: SH-SY5Y whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 20 kDa
Rheb Rabbit Polyclonal Antibody
Rheb Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Rheb |
| Synonyms | GTP-binding protein Rheb; Ras homolog enriched in brain; RHEB2; RHEB |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Lysosome, Endoplasmic reticulum |
| Accession | Q15382 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | A549, U-87 MG, SH-SY5Y |
| Predicted Reactivity | Bv, Dr, Ms, Rt |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:200 | Hu |
Background
Rheb protein is a small GTPase that plays a crucial role in regulating cell growth and metabolism. It acts as a key activator of the mTOR (mechanistic target of rapamycin) pathway, which is central to controlling protein synthesis, cell size, and autophagy. When Rheb is bound to GTP, it stimulates mTORC1 activity, promoting anabolic processes that support cell growth and proliferation. Conversely, when it is in its GDP-bound state, mTORC1 is inhibited, allowing cells to shift towards catabolic processes. Rheb is involved in various cellular functions and its dysregulation has been implicated in several diseases, including cancer and neurodegenerative disorders.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-Rheb antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-Rheb antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-Rheb antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Rheb antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Rheb antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
