WB result of RFX5 Recombinant Rabbit mAb
Primary antibody: RFX5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Lane 4: Raji whole cell lysate 20 µg
Lane 5: HCT 116 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 75 kDa
RFX5 Recombinant Rabbit mAb (S-3628-50)
RFX5 Recombinant Rabbit mAb (S-3628-50)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | RFX5 |
| Synonyms | DNA-binding protein RFX5; Regulatory factor X 5 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P48382 |
| Clone Number | S-3628-50 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | HeLa, Jurkat, K562, Raji, HCT 116 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:500 | Hu |
Background
The RFX5 protein is a core subunit of a key transcription factor complex regulating adaptive immunity; its canonical function involves assembling with RFXAP and RFXANK into a heterotetramer (an RFX5 dimer bound to one RFXAP and one RFXANK molecule), recognizing the X-box motifs in MHC-I and MHC-II promoters via its N-terminal conserved domain, and utilizing its C-terminal domain to collaborate with NF-Y and other factors in stabilizing transcriptional enhanceosomes—deficiencies in this function lead to primary immunodeficiencies such as bare lymphocyte syndrome (BLS). The latest research revealed at the structural level that RFX5 also acts as a unique pioneer transcription factor: its extended DNA-binding domain (eDBD) directly forms hydrogen bonds with histones via the Glu102 residue, specifically targeting the SHL+2 position on nucleosomes. This induces localized DNA distortion and the dissociation of distal DNA from the nucleosome surface, thereby actively opening chromatin to enhance DNA accessibility and paving the way for subsequent transcription factor binding.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-RFX5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-RFX5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-RFX5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-RFX5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
