WB result of RBM15B Recombinant Rabbit mAb
Primary antibody: RBM15B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: 293T whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 97 kDa
Observed MW: 100 kDa
RBM15B Recombinant Rabbit mAb (S-1326-111)
RBM15B Recombinant Rabbit mAb (S-1326-111)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | RBM15B |
| Synonyms | Putative RNA-binding protein 15B; One-twenty two protein 3 (HsOTT3; HuOTT3); RNA-binding motif protein 15B; OTT3 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | Q8NDT2 |
| Clone Number | S-1326-111 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | HeLa, Jurkat, 293T |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:250 | Hu |
Background
RBM15B, also known as RNA binding motif protein 15B, is a member of the SPEN (Split-end) family of proteins and is involved in various cellular processes. It functions as an RNA-binding protein that plays a key role in regulating N6-methyladenosine (m6A) methylation of RNAs, which affects mRNA splicing and processing. RBM15B is also implicated in the regulation of alternative splicing and acts as a cofactor for the nuclear export receptor NXF1, influencing mRNA export from the nucleus. Additionally, it has been associated with diseases such as Tatton-Brown-Rahman Syndrome and Wilms Tumor 1, highlighting its importance in both normal and pathological cellular functions.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human stomach. Anti-RBM15B antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-RBM15B antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-RBM15B antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
