RBBP4 Rabbit Polyclonal Antibody

Catalog Number: S0B60006 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	RBBP4
Synonyms	Histone-binding protein RBBP4; Chromatin assembly factor 1 subunit C (CAF-1 subunit C); Chromatin assembly factor I p48 subunit (CAF-I 48 kDa subunit; CAF-I p48); Nucleosome-remodeling factor subunit RBAP48; Retinoblastoma-binding protein 4 (RBBP-4); Retinoblastoma-binding protein p48; RBAP48
Immunogen	Synthetic Peptide
Location	Nucleus
Accession	Q09028
Antibody Type	Polyclonal antibody
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	SH-SY5Y, HeLa Jurkat, HEK-293, mouse spleen, F9, rat spleen
Purification	Immunogen Affinity
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:2000	Hu, Ms, Rt
IHC-P	1:1000	Hu, Ms, Rt

Background

RBBP4 (Retinoblastoma Binding Protein 4), also known as RbAp48 or NURF55, is a highly conserved core subunit of several essential chromatin-modifying complexes, including the NuRD (Nucleosome Remodeling and Deacetylase) complex, the PRC2 (Polycomb Repressive Complex 2), and the HAT1 histone acetyltransferase complex. Functioning primarily as a histone chaperone, RBBP4 facilitates the assembly and stability of nucleosomes by binding directly to the N-terminal tails of histones H3 and H4, thereby playing a pivotal role in regulating chromatin structure and epigenetic gene expression. Through its involvement in these multiprotein complexes, RBBP4 contributes to critical cellular processes such as transcriptional repression, DNA replication, cell cycle progression, and cellular differentiation, while its dysregulation has been implicated in various pathological conditions, including cancer and neurodegenerative disorders, highlighting its significance as both a fundamental regulator of genome integrity and a potential therapeutic target.

Picture

Western Blot

WB result of RBBP4 Rabbit pAb
Primary antibody: RBBP4 Rabbit pAb at 1/1000 dilution
Lane 1: SH-SY5Y whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 50 kDa

WB result of RBBP4 Rabbit pAb
Primary antibody: RBBP4 Rabbit pAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Lane 3: F9 whole cell lysate 20 µg
Low expression control: mouse brain lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 50 kDa

WB result of RBBP4 Rabbit pAb
Primary antibody: RBBP4 Rabbit pAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Lane 2: rat spleen lysate 20 µg
Low expression control: rat brain lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 50 kDa

WB result of RBBP4 Rabbit pAb
Primary antibody: RBBP4 Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 50 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human kidney. Anti-RBBP4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver. Anti-RBBP4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-RBBP4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-RBBP4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-RBBP4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse kidney. Anti-RBBP4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-RBBP4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.