Product Details
Product Details
Product Specification
| protein | IL-6 |
| Species Reactivity | Rat |
| Source | Rat |
| Description |
Detection principle: This kit uses double antibody sandwich ELISA Technologies. Specificity anti- Rat IL-6 capture antibody Pre-coated on high affinity enzyme plates. The standard substance and the sample to be tested are sequentially added to the well of the enzyme label plate , Thoroughly shake and mix, and then put it at room temperature 2 Hours of incubation process, present in the sample IL-6 Binding to solid phase antibodies. After thorough washing to remove free and unbound components, biotinylated detection antibody is added for incubation. After washing again, horseradish peroxidase-labeled streptavidin was added (Streptavidin-HRP , SA-HRP) 。 After washing, the signal enhancer was added for incubation, and after washing to remove unbound material, it was added again SA-HRP 。 After washing again, add TMB The chromogenic substrate is incubated at room temperature in the dark from light to develop color. The depth of color reaction and the sample IL-6 The concentration is positively correlated. Add a stop solution to stop the reaction, and use a microplate reader to 450 nm Detection wavelength ( Correction wavelength 570-630 nm) The absorbance values were determined under conditions.
Test Type: Double antibody sandwich method
Form: Pre-coating 96 Orifice plate
Test Sample Type: Cell supernatant, serum, plasma
Sample Load: 100 μl
Kit Components: Pre-coating 96 Well plate, standard, biotin labeled IL-6 Test antibody, standard dilution, test buffer, signal enhancer, signal enhancer dilution, TMB A chromogenic substrate, a wash liquid, a stop liquid, SA-HRP , a copy of the sealing film and instructions.
Sensitivity: 1.80 pg/mL
Detection range: 7.81-500 pg/mL
Recovery Range: 95-106%
Save Method: 2-8℃
Standard graph:
Background: Interleukin 6(IL-6) It is a cytokine with both pro-inflammatory and anti-inflammatory properties. In humans, it is composed of IL-6 Genetic encoding. During infection and after trauma, especially inflammation caused by burns or other tissue damage, IL-6 Will be by T Cells and macrophages secrete, promoting the immune response. Studies have shown that mice need to resist Streptococcus pneumoniae infection IL-6 Participation, describing IL-6 Plays a role in fighting infections. IL-6 Inflammatory and autoimmune disease processes can be promoted in many diseases such as diabetes, atherosclerosis, depression, Alzheimer's disease, systemic lupus erythematosus, multiple myeloma, prostate cancer, Behcet's disease and rheumatoid arthritis. later period / Patients with metastatic cancer have higher levels in their blood IL-6 。 |

