Rat IL-6 ELISA Kit
Rat IL-6 ELISA Kit
Price:
Regular price
$450 USD
Regular price
Sale price
$450 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Species Reactivity | Rat | |||||||||||||||||||||||||||
| Theory | This kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-rat IL-6 antibodies were pre-coated on high affinity enzyme plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, IL-6 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm). | |||||||||||||||||||||||||||
| Synonym | B cell stimulation factor-2, B-cell differentiation factor, BSF2, BSF-2, BSF2CTL differentiation factor, CDF, HGFHSFIFNB2Hybridoma growth factor, IFNB2, IFN-beta-2, IL6, IL-6, IL-6B-cell stimulation factor 2, interferon beta-2, interleukin 6 (interferon, beta 2), interleukin BSF-2, interleukin-6, MGI-2A, Rat interleukin 6 Elisa kit | |||||||||||||||||||||||||||
| Composition |
Please use it within the validity period of the kit (new and old products are shipped randomly)
|
|||||||||||||||||||||||||||
| Background | Interleukin -6 IL-6 ) is a system with α A spiral structure, 22-28kDa It plays an important role in the acute phase of disease, inflammation, hematopoiesis, bone metabolism and cancer exacerbation. A mature person IL-6 have 183 Amino acids, with mice and rats IL-6 have 41% Homology of. IL-6 Internal alternative splicing results in a variety of isomers, some of which exhibit antagonistic properties. Known expression IL-6 The cells comprise CD8+T Cells, fibroblasts, synoviocytes, adipocytes, osteoblasts, megakaryocytes, endothelial cells (under the influence of endothelin), sympathetic neurons, cerebral cortical nerve cells, adrenal medullary chromaffin cells, retinal pigment cells, mast cells, keratinocytes, Langerhans cells, fetal and adult astrocytes, neutrophils, monocytes, eosinophils, colonic epithelial cells, B1B β Cells. IL-6 The production is usually under the control of glucocorticoids, catecholamines and secondary sex steroids and is generally associated with cellular activation. In normal human blood IL-6 In 1pg/mL Within the range, there is a slight increase in menstrual period, a moderate increase in some cancers, and a significant increase after surgery. IL-6 Cellular signaling is initiated by a cell surface heterodimeric receptor complex consisting of a ligand-binding subunit （ IL-6 Receptor) and a signal transduction subunit gp130 Composition. IL-6 Binding to IL-6 IL-6 Receptor with gp130 Combination of and gp130 Dimerization of. gp130 Also CLC CNTF 、 CT-1 、 IL-11 IL-27 、 LIF And OSM IL-6 Receptors are produced by alternative splicing and proteolysis. Through a trans-signaling mechanism, soluble IL-6 IL-6 Receptor complexes can trigger surface deficiency IL-6 gp130 Response of cells. IL-6 gp130 Molecular expression is very broad, and trans-signaling enables a wider range of cell type pairs IL-6 Response of. Soluble gp130 The spliceosome prevents IL-6/IL-6R Trans-signal transduction, but does not prevent other cytokine utilization gp130 Signal transduction of molecules as co-receptors. IL-6 With tumor necrosis factor ( TNF ) and IL-1 The acute inflammatory reaction caused by it plays an almost unique role in fever and acute inflammatory reaction of the liver, and it also plays an important role in the transformation of acute inflammation into acquired immunity or chronic inflammatory diseases. IL-6 Disorders contribute to chronic inflammation in cases such as obesity, insulin resistance, inflammatory bowel disease, inflammatory arthritis, and sepsis, often involving IL-6 TGF- Under the conditions of existence, IL-6 In the naive type T Cells to Th17 It plays an important role in the process of inflammatory cell differentiation. IL-6 Modulates bone resorption, and by promoting Th17 The active component of inflammatory cells is the main factor causing inflammatory joint damage in rheumatoid arthritis I 。 L-6 Contributes to the formation and instability of atherosclerotic plaque. but IL-6 It also has anti-inflammatory effects, such as skeletal muscle secretion during physical exercise IL-6 。 It acts as a hematopoietic stem cell growth factor to promote hematopoiesis, induction B The cells mature into plasma cells, immortalized multiple myeloma cells. IL-6 It also promotes but initiates the development of other inflammation-related cancers, such as enteritis-related cancers. |
|||||||||||||||||||||||||||
| General Notes | 1. Please use the kit within the validity period. 2. The components of different kits and different batch kits cannot be mixed. 3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step. 4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc. 5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it. 6. For scientific research only, not for in vitro diagnosis. |
|||||||||||||||||||||||||||
| Storage Temp. | Unopened kit, store at 2-8°C | |||||||||||||||||||||||||||
| Test Range | 125pg/mL-8000pg/mL |
Picture
Picture
Immunohistochemistry
