Standard curve
Example of Rat IGF-1 standard curve in Assay Diluent #5.
Rat IGF-1 OneStep ELISA Kit
Rat IGF-1 OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | IGF-1 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Rt |
| Purification | Protein A |
| Stability & Storage | 2 to 8 °C as supplied. |
Kit
| Precision | Intra-assay: 5.6%； Inter-assay: 7.2% |
| Sample type | Cell culture supernatant, Serum |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 4 pg/ml |
| Range | 6.25 pg/mL – 400 pg/mL |
| Recovery | Cell culture supernatant: 106% Serum: 96% Plasma: 115% |
| Assay time | 60 minutes |
| Species reactivity | Rat |
Background
Rat Insulin-like Growth Factor-1 (IGF-1) is a key regulatory protein involved in growth, metabolism, and cellular repair. Structurally homologous to human and mouse IGF-1, it binds to the IGF-1 receptor (IGF-1R) to activate downstream signaling pathways such as PI3K/Akt and MAPK, promoting cell proliferation, differentiation, and survival. Rat IGF-1 is widely utilized in research to investigate growth disorders, metabolic diseases, tissue regeneration, and aging processes in rat models. Recombinant rat IGF-1, produced in E. coli or mammalian expression systems, ensures high biological activity and consistency for experimental applications. It is particularly valuable in studies related to diabetes, neuroprotection, muscle development, and wound healing.
Picture
Picture
ELISA
Linearity
The concentrations of Rat IGF-1 were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: Rat Serum was 1x10^3 fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 692.1 ng/mL in Rat Serum.
To assess the linearity of the assay, sample was spiked with high concentrations of Rat IGF-1 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
To assess the linearity of the assay, two samples were spiked with high concentrations of Rat IGF-1 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Protocol Diagram
