Flow cytometric analysis of SD rat splenocytes labeled with Rat CD32 antibody at 1/50 dilution (1 μg) / (right panel) compared with a Mouse IgG1, κ Isotype Control / (left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD45R/B220 – PE antibody separately. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Rat FcR Blocking Reagent
Rat FcR Blocking Reagent
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$60 USD
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD32 |
| Synonyms | anti-rat CD32 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM, Fc receptor blocking |
| Reactivity | Rt |
| Positive Sample | SD rat splenocytes |
| Purification | Protein G |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| Fc receptor blocking | 1μg (2μl/test) per million cells in 100μl volume | Rt |
Background
FcR Blocking Reagent is a specialized reagent used in flow cytometry experiments to prevent non-specific binding between the Fc segment of antibodies and Fc receptors (FcRs) on the cell surface. This is crucial to avoid false positive or negative results in immunofluorescence staining. Human FcR Blocking Reagent is compatible with flow cytometric staining with anti-human CD16, CD32, and CD64 antibodies.
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