Standard Curve
Example of Rat EGF standard curve in Assay Diluent HS2. Plotted are the background-subtracted data.
This standard curve is provided for demonstration only.
Rat EGF OneStep ELISA Kit
Rat EGF OneStep ELISA Kit
Price:
Regular price
$500 USD
Regular price
Sale price
$500 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Antigen | EGF |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Rat |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8°C as supplied. |
Kit
| Precision | Intra-assay: 3.0%； Inter-assay: 4.3% |
| Sample type | Serum; EDTA Plasma, Heparin Plasma, Citrate Plasma; Cell Culture Supernatant |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.49 pg/mL |
| Range | 2.3 pg/mL –150 pg/mL |
| Recovery | SD Serum: 94% SD EDTA Plasma: 112% SD Citrate plasma: 103% SD Heparin Plasma: 99% Lewis Serum: 98% Lewis EDTA Plasma: 101% Lewis Citrate plasma: 96% Lewis Heparin Plasma: 100% Cell Culture Supernatant: 107% |
| Assay time | 60 minutes |
| Species reactivity | Rat |
Background
Epidermal Growth Factor (EGF) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. EGF binds to epidermal growth factor receptor and stimulates an intracellular signal transduction cascade, leading to activation of genes that regulate cell proliferation, angiogenesis, motility, and metastasis. EGF mRNA and protein are expressed in a number of adult tissues, especially in epithelial cells in the gastrointestinal tract. Predominant sites of synthesis of this peptide are the submandibular glands, the Brunner glands in the small intestine and the kidney.
Picture
Picture
ELISA
Spike Recovery
The recovery of Rat EGF was evaluated in activated samples spiked with concentrations spanning the entire assay range.
Spike-and-dilution Linearity
To evaluate assay linearity, samples were spiked with high levels of Rat EGF in different matrices and serially diluted with the corresponding Calibrator Diluent to fall within the assay's dynamic range.
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested fifteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays).
Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed with at least three lots of components.
Determination of Minimum Detectable Dose (MDD)
The MDD was determined using three independent lots of assay components. For each lot, 19 replicate measurements of the diluent (zero calibrator) were performed. The mean (AVERAGE) and standard deviation (STDEV) of the 19 replicates were calculated. The MDD for each lot was then calculated according to the following formula:
MDD = 2 × STDEV + AVERAGE
Cross-reactivity
The cross-reactivity between Rat EGF, Mouse EGF and Human EGF was assessed by testing both proteins in the same assay system. Serial dilutions of each protein were measured, and the dose-response curves were compared. No significant cross-reactivity was observed when Mouse EGF and Human EGF were tested in the Rat EGF assay, as the signals remained within the background range of the assay.
HOOK Effect Threshold
The upper limit of the HOOK effect was established at 100× the highest calibrator concentration (equivalent to a 2-log10 increase). No HOOK effect was observed below this threshold, confirming that samples within this concentration range yield reliable quantitative results without signal depression.
Samples in healthy individuals
Duplicate measurements were performed on samples obtained from eight individual healthy Rats. Data are reported as dilution factor-corrected interpolated. The resultant mean EGF concentration was 18.6 pg/mL, with a distribution spanning 10.7 to 28.1 pg/mL.
Protocol Diagram
