Flow cytometric analysis of Fresh C57BL/6 mouse splenocytes labelling Mouse Siglec-H antibody at 1/200 dilution (1 μg) / (Right panel) compared with a Rat IgG2b Isotype Control / (Left panel). Goat Anti-Rat IgG Alexa Fluor® 647 was used as the secondary antibody. Then cells were stained with CD45R/B220 - Pacific Blue™ Antibody separately.
Rat Anti-Mouse Siglec-H Antibody (S-3779)
Rat Anti-Mouse Siglec-H Antibody (S-3779)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | Siglec-H |
| Synonyms | Siglec-H; Siglech |
| Location | Membrane |
| Accession | Q3Y597 |
| Clone Number | S-3779 |
| Antibody Type | Rat mAb |
| Isotype | IgG2b |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | Fresh C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1:200 | Ms |
Background
Siglec-H is a sialic acid-binding Ig-like lectin expressed almost exclusively on murine plasmacytoid dendritic cells (pDCs) and microglia, where it partners with the ITAM-bearing adaptor DAP12 to deliver inhibitory signals that restrain type I interferon (IFN-α) secretion, limits TLR7/9-mediated viral responses, and thereby prevents overt systemic autoimmunity while still permitting antigen uptake and presentation; genetic deletion or antibody-mediated ligation of Siglec-H heightens IFN-α output, skews T-cell polarization toward IL-21⁺ CD4 subsets, and can either exacerbate or attenuate inflammatory diseases depending on context, making it a key rheostat of innate–adaptive immune crosstalk.
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