Flow cytometric analysis of BALB/c mouse splenocytes labelling Mouse Siglec-E antibody at 1/200 dilution (1 μg) / (Right panel) compared with a Rat IgG2b Isotype Control / (Left panel). Goat Anti-Rat IgG Alexa Fluor® 647 was used as the secondary antibody. Then cells were stained with LY6G/LY6C - Pacific Blue™ Antibody separately.
Rat Anti-Mouse Siglec-E Antibody (S-3881)
Rat Anti-Mouse Siglec-E Antibody (S-3881)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | Siglec-E |
| Synonyms | Sialic acid binding Ig-like lectin E; Siglece; Siglecl1 |
| Location | Membrane |
| Accession | Q91Y57 |
| Clone Number | S-3881 |
| Antibody Type | Rat mAb |
| Isotype | IgG2b |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | BALB/c mouse splenocytes |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1:200 | Ms |
Background
Siglec-E, the murine orthologue of human Siglec-9, is a sialic acid-binding immunoglobulin-like lectin expressed predominantly on myeloid cells (macrophages, neutrophils, dendritic cells) that functions as a critical immunosuppressive checkpoint by recruiting SHP-1/2 phosphatases upon engagement of sialoglycan ligands, thereby attenuating TLR4- and TRIF-dependent signaling, dampening NF-κB activation and pro-inflammatory cytokine production, and exerting neuroprotective and anti-atherogenic effects through inhibition of microglial activation and CD36-mediated oxidized LDL uptake.
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