WB result of PD-1 (mouse)Rat mAb
Primary antibody: PD-1(mouse) Rat mAb at 1/1000 dilution
Lane 1: recombinant PD-1 Fc Chimera 1 µg
Secondary antibody: Goat Anti-Rat IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 50~70 kDa
(This blot was developed with high sensitivity substrate)
Rat-Anti Mouse PD-1 Antibody (S-798-5)
Rat-Anti Mouse PD-1 Antibody (S-798-5)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | PD-1 |
| Synonyms | Programmed cell death protein 1, Protein PD-1, mPD-1, CD279, Pdcd1 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | Q02242 |
| Clone Number | S-798-5 |
| Antibody Type | Rat mAb |
| Isotype | IgG1,k |
| Application | WB, ICC, FCM |
| Reactivity | Ms |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| ICC | 1:100 | |
| FCM | 1:2000 |
Background
Programmed cell death protein 1, also known as PD-1 and CD279 (cluster of differentiation 279), is a protein on the surface of T and B cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self-tolerance by suppressing T cell inflammatory activity. This prevents autoimmune diseases, but it can also prevent the immune system from killing cancer cells. PD-1 is an immune checkpoint and guards against autoimmunity through two mechanisms. First, it promotes apoptosis (programmed cell death) of antigen-specific T-cells in lymph nodes. Second, it reduces apoptosis in regulatory T cells (anti-inflammatory, suppressive T cells).
Picture
Picture
Western Blot
FC
Flow cytometric analysis of NIH/3T3 (Mouse embryonic fibroblast, left) / EL4.IL-2 (Mouse lymphoma T lymphocyte, Right) labelling PD-1 (mouse) antibody at 1/2000 dilution (0.1 μg) / (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti – Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Negative control: NIH/3T3
Immunocytochemistry
ICC shows positive staining in EL4.IL2 cells. Anti- PD-1 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rat IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
Negative control: ICC shows negative staining in NIH/3T3 cells. Anti- PD-1 antibody was used at 1/100 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rat IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
