Flow cytometric analysis of C57BL/6 mouse splenocytes labelling Mouse Ly-6A/E antibody at 1/2000 (0.1 μg) dilution/ (right panel) compared with a Rat IgG2a, κ Isotype Control / (left panel). Goat Anti-Rat IgG Alexa Fluor® 488 was used as the secondary antibody.
Rat Anti-Mouse Ly-6A/E Antibody (S-3835)
Rat Anti-Mouse Ly-6A/E Antibody (S-3835)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | Ly-6A/E |
| Synonyms | Lymphocyte antigen 6A-2/6E-1; Ly-6A.2/Ly-6E.1; Stem cell antigen 1 (SCA-1); T-cell-activating protein (TAP) |
| Location | Cell membrane |
| Accession | P05533、Q64253 |
| Clone Number | S-3835 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1:2000 | Ms |
Background
Ly-6A/E, also known as Sca-1 (stem cell antigen-1), is an 18-kDa glycosylphosphatidylinositol (GPI)-anchored cell surface protein belonging to the murine Ly-6 multigene family, predominantly expressed on multipotent hematopoietic stem cells in adult mouse bone marrow and fetal liver, and is widely used as a marker for isolating these stem cells; beyond its stem cell association, Ly-6A/E is upregulated on activated T and B lymphocytes and appears to participate in T-cell receptor-mediated activation, myoblast proliferation and differentiation, bone formation, and interferon-induced immune responses, with expression levels varying between mouse strains carrying different Ly-6 haplotypes.
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