Flow cytometric analysis of 4% PFA fixed and Fixation/Permeabilization solution permeabilized Th1 polarized C57BL/6 mouse splenocytes, stimulated 5h with PMA and Ionomycin in the presence of Brefeldin A (Right panel) or untreated (Left panel) labelling mouse IFN-γ antibody at 1/200 dilution (1 μg). Goat Anti - Mouse IgG Alexa Fluor® 647 was used as the secondary antibody.
Rat Anti-Mouse IFNγ Antibody (XMG1.2)
Rat Anti-Mouse IFNγ Antibody (XMG1.2)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | IFNγ |
| Synonyms | Interferon gamma; IFN-gamma; Ifng |
| Location | Secreted |
| Accession | P01580 |
| Clone Number | XMG1.2 |
| Antibody Type | Rat mAb |
| Isotype | IgG1,k |
| Application | ICFCM |
| Reactivity | Ms |
| Positive Sample | Th1 polarized C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICFCM | 1:200 | Ms |
Background
Interferon-gamma (IFNγ) is the signature cytokine of type II interferon, a 20–25 kDa glycoprotein homodimer that is synthesized and secreted primarily by activated T cells, NK cells and NKT cells; upon binding to its high-affinity receptor (IFNGR1/2) it triggers JAK1/JAK2-mediated phosphorylation of STAT1, launching a transcriptional program that up-regulates MHC class I and II, antigen-processing machinery, chemokines such as CXCL9/10/11, nitric-oxide and reactive-oxygen species, and hundreds of IFN-stimulated genes, thereby polarizing macrophages toward the microbicidal M1 phenotype, enhancing Th1 responses, orchestrating antiviral, antitumor and intracellular bacterial immunity, and exerting pro- or anti-inflammatory feedback depending on context; genetic or therapeutic blockade of IFNγ in mice and humans highlights its essential yet double-edged role in controlling infection, tumor surveillance, autoimmune diseases and immune-related adverse events.
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