Flow cytometric analysis of C57BL/6 mouse splenocytes labelling Mouse CD9 antibody at 1/2000 (0.1 μg) dilution. Goat Anti-Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with Ly-6G/Ly-6C - Pacific Blue™ Antibody separately.
Rat Anti-Mouse CD9 Antibody (S-R652)
Rat Anti-Mouse CD9 Antibody (S-R652)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | Mouse CD9 |
| Synonyms | CD9 antigen |
| Location | Cell membrane |
| Accession | P40240 |
| Clone Number | S-R652 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 1:2000 | Ms |
Background
CD9 is a member of the tetraspanin family, a type of cell surface glycoprotein characterized by four transmembrane domains and two extracellular loops with conserved disulfide bonds. It plays a role in various cellular processes, including cell adhesion, signal transduction, and the suppression of cancer cell motility and metastasis. CD9 is also involved in immune responses, such as the activation of the PI3K/AKT signaling pathway in B-lineage acute lymphoblastic leukemia. Additionally, it has been implicated in the regulation of extracellular vesicle secretion and is a potential biomarker for several cancers.
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