Flow cytometric analysis of C57BL/6 mouse splenocytes labeled anti-mouse CD45R/B220 antibody at 1/200 dilution (1 μg) (right panel) compared to a Rat IgM, κ Isotype Control (left panel). Goat Anti-Rat IgM Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD3 - Brilliant Violet 421™ antibody separately. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Rat Anti-Mouse CD45R/B220 Antibody (RA3.3A1/6.1)
Rat Anti-Mouse CD45R/B220 Antibody (RA3.3A1/6.1)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD45R/B220 |
| Synonyms | Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen (L-CA); Lymphocyte antigen 5 (Ly-5); T200; CD45; Ly-5; Ptprc |
| Location | Cell membrane, Synapse |
| Accession | P06800 |
| Clone Number | RA3.3A1/6.1 |
| Antibody Type | Rat mAb |
| Isotype | IgM,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1:200 | Ms |
Background
CD45R/B220 is an isoform of the CD45 protein, a member of the protein tyrosine phosphatase (PTP) family, and serves as a critical regulator of T- and B-cell antigen receptor signaling. It is predominantly expressed on the surface of B cells, from early pro-B cells to mature and activated B cells, making it a commonly used pan-B cell marker in mice. Additionally, CD45R/B220 is also found on certain subsets of activated T cells, lymphokine-activated killer cells (LAK), NK cell progenitors, and T cells in mutant mice. This molecule plays a crucial role in lymphocyte development and antigen signaling, and its expression is regulated during different stages of B cell maturation.
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