Flow cytometric analysis of bEnd.3 (Mouse brain endothelioma, Right) / NIH/3T3 (Mouse embryonic fibroblast, Left) cells labelling CD309 antibody at 1/200 dilution (1 μg)/ (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rat IgG PE was used as the secondary antibody.
Negative control: NIH/3T3
Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD309 |
| Synonyms | Vascular endothelial growth factor receptor 2; VEGFR-2; Fetal liver kinase 1 (FLK-1); Kinase NYK; Protein-tyrosine kinase receptor flk-1; Flk-1; Flk1; Kdr |
| Location | Cell junction |
| Accession | P35918 |
| Clone Number | S-R706 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | bEnd.3 |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1:200 | Ms |
Background
CD309, also known as VEGFR-2 or KDR, is a type III receptor tyrosine kinase that functions as the primary mediator of VEGF-induced angiogenesis, endothelial cell proliferation, migration, survival, and vascular permeability by activating downstream signaling pathways including MAPK, PI3K-Akt, and PLCγ1 upon ligand binding.
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