Flow cytometric analysis of IL-10 expression in Human PBMC polarized with Th2 Polarization Kit, Human (UA090018). Th2 polarized human PBMC were harvested and restimulated for 5 hr with PMA and Ionomycin in the presence of Brefeldin A (right panel) or unstimulated (left anel).The cells were harvested and fixed with 4% PFA and permeabilized with Intracellular Fixation & Permeabilization Buffer Set. The cells were then labeled Human IL-10 antibody at 1/200 dilution (1 μg). Goat Anti-Rat IgG Alexa Fluor® 647 was used as the secondary antibody. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Rat Anti-Human IL-10 Antibody (S-3081)
Rat Anti-Human IL-10 Antibody (S-3081)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | IL-10 |
| Synonyms | Interleukin-10; IL-10; Cytokine synthesis inhibitory factor (CSIF); IL10 |
| Location | Secreted |
| Accession | P22301 |
| Clone Number | S-3081 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | ICFCM |
| Reactivity | Hu |
| Positive Sample | Th2 polarized human PBMC |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICFCM | 1:200 | Hu |
Background
Interleukin-10 (IL-10) is a cytokine that plays a crucial role in regulating immune responses. It is primarily produced by various immune cells, including monocytes, macrophages, and regulatory T cells. IL-10 has potent anti-inflammatory properties, as it inhibits the production of pro-inflammatory cytokines such as IL-1, TNF-α, and IFN-γ from activated macrophages and T helper 1 cells. By doing so, it helps to prevent excessive inflammation and tissue damage. Additionally, IL-10 promotes the differentiation of regulatory T cells and enhances the function of B cells, contributing to the maintenance of immune homeostasis. Dysregulation of IL-10 production has been implicated in various diseases, including autoimmune disorders and certain types of cancer, highlighting its importance in both health and disease.
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