WB result of RanBP2 Recombinant Rabbit mAb
Primary antibody: RanBP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: SH-SY5Y whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 358 kDa
Observed MW: 370 kDa
RanBP2 Recombinant Rabbit mAb (S-2593-310)
RanBP2 Recombinant Rabbit mAb (S-2593-310)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | RanBP2 |
| Synonyms | E3 SUMO-protein ligase RanBP2; 358 kDa nucleoporin; Nuclear pore complex protein Nup358; Nucleoporin Nup358; Ran-binding protein 2 (RanBP2); p270; NUP358; RANBP2 |
| Immunogen | Recombinant Protein |
| Location | Nucleus |
| Accession | P49792 |
| Clone Number | S-2593-310 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | HeLa, 293T, SH-SY5Y |
| Predicted Reactivity | Cz |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:1000 | Hu |
Background
RanBP2, a 358-kDa nucleoporin that forms the cytoplasmic filaments of the nuclear pore complex, functions as an E3 SUMO ligase and a scaffold for RanGAP1-SUMO1-Ubc9 complexes, thereby orchestrating nucleocytoplasmic transport, mitotic spindle assembly, and the recruitment of kinesin-1 to drive plus-end-directed motility of aggresomes and lipid droplets while also regulating NF-κB signaling, circadian rhythms, and neuronal responses through its extensive network of interactions at the pore and beyond.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human placenta. Anti-RanBP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-RanBP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-RanBP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-RanBP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-RanBP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
