Rad51C Recombinant Rabbit mAb (S-4505-32)

Catalog Number: S0B60543 Application: WB, IHC-P Reactivity: Hu Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	Rad51C
Immunogen	Synthetic Peptide
Location	Cytoplasm, Nucleus, Mitochondrion
Accession	O43502
Clone Number	S-4505-32
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu
Positive Sample	HCT 116, MCF7, A-172, MDA-MB-231, HEK-293
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu
IHC-P	1:500-1:2000	Hu

Background

RAD51C (RAD51 paralog C) is a key DNA homologous recombination repair protein belonging to the RAD51 paralog gene family. It was initially discovered in 1998 through computational screening of EST databases and encodes a protein of 376 amino acids with 18–26% amino acid homology to other human Rad51 family members. In cells, RAD51C forms two functional complexes: the BCDX2 complex (containing RAD51B, RAD51C, RAD51D, and XRCC2) and the CX3 complex (containing RAD51C and XRCC3). These complexes assist in loading the recombinase RAD51 onto single-stranded DNA during homologous recombination repair of DNA double-strand breaks, promoting the formation of RAD51 nucleoprotein filaments, which is the basis for the homology search and strand invasion steps. RAD51C also participates in DNA replication fork protection and restart, and its Walker A and Walker B motifs are essential for ATP binding and hydrolysis. Recent studies have further identified an ovarian cancer-associated mutation cluster in the Walker B region and characterized separation-of-function alleles that uncouple homologous recombination from replication functions. Clinically, germline pathogenic variants in RAD51C are associated with hereditary breast and ovarian cancer susceptibility syndrome type 3 (BROVCA3) and Fanconi anemia complementation group O. Ovarian cancer risk in RAD51C variant carriers is comparable to that in BRCA2 carriers (odds ratios of 8.3 and 9.94, respectively), and tumors often harbor loss of heterozygosity and p53 defects. RAD51C-deficient tumors show sensitivity to PARP inhibitors (such as olaparib) and platinum-based agents (such as cisplatin), but loss of promoter methylation or reversion mutations can lead to acquired resistance.

Picture

Western Blot

WB result of Rad51C Recombinant Rabbit mAb
Primary antibody: Rad51C Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HCT 116 whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: A-172 whole cell lysate 20 µg
Lane 4: MDA-MB-231 whole cell lysate 20 µg
Lane 5: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 38 kDa
This blot was developed with high sensitivity substrate

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-Rad51C antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human spleen. Anti-Rad51C antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-Rad51C antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Rad51C antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-Rad51C antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human stomach cancer. Anti-Rad51C antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.