RAD21 Recombinant Rabbit mAb (S-4271-136)

Catalog Number: S0B60680 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	RAD21
Synonyms	Double-strand-break repair protein rad21 homolog; Nuclear matrix protein 1 (NXP-1); SCC1 homolog; HR21; KIAA0078; NXP1; SCC1
Immunogen	Synthetic Peptide
Location	Nucleus
Accession	O60216
Clone Number	S-4271-136
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	Hela, Jurkat, K-562, HEK-293, HT-29, MOLT-4, NIH/3T3, C2C12, mouse spleen, C6
Predicted Reactivity	Hm, Fs, Cm
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms, Rt
IHC-P	1:500	Hu, Ms, Rt

Background

RAD21 (also known as SCC1 or NXP-1) is the core cleavable subunit of the cohesin complex, composed of 628 amino acids and highly conserved in eukaryotes. Its N-terminus binds to the SMC3 head, while its C-terminus binds to the SMC1A head, bridging these two SMC proteins to form an asymmetric ring structure of approximately 40 nm that entraps sister chromatids. During the cell cycle, RAD21 maintains sister chromatid cohesion from S phase through mitosis, ensuring proper chromosome segregation and post-replicative DNA double-strand break repair. Upon entry into anaphase, RAD21 is cleaved by separase, causing cohesin to dissociate from chromosomes and allowing sister chromatids to separate. Beyond its canonical cohesion function, RAD21 also participates in gene expression regulation by binding specific genomic loci during interphase to modulate RNA polymerase II transcription. Dysregulation of RAD21 is closely associated with multiple diseases: germline mutations can cause Cornelia de Lange syndrome (CdLS4 type), a developmental disorder characterized by facial dysmorphism, growth retardation, and cognitive impairment, while somatic dysregulation promotes malignant progression in various cancers, including colorectal, breast, and prostate cancer, where its overexpression often correlates with aggressive phenotypes, chemoresistance, and poor prognosis.

Picture

Western Blot

WB result of RAD21 Recombinant Rabbit mAb
Primary antibody: RAD21 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: K-562 whole cell lysate 20 µg
Lane 4: HEK-293 whole cell lysate 20 µg
Lane 5: HT-29 whole cell lysate 20 µg
Lane 6: MOLT-4 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 71 kDa
Observed MW: 125, 67 kDa
This blot was developed with high sensitivity substrate

WB result of RAD21 Recombinant Rabbit mAb
Primary antibody: RAD21 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: C2C12 whole cell lysate 20 µg
Lane 3: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 71 kDa
Observed MW: 125, 67 kDa
This blot was developed with high sensitivity substrate

WB result of RAD21 Recombinant Rabbit mAb
Primary antibody: RAD21 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 71 kDa
Observed MW: 125, 67 kDa
This blot was developed with high sensitivity substrate

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human placenta. Anti-RAD21 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human testis. Anti-RAD21 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon. Anti-RAD21 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-RAD21 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-RAD21 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human pancreas cancer. Anti-RAD21 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse testis. Anti-RAD21 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse lung. Anti-RAD21 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat kidney. Anti-RAD21 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat lung. Anti-RAD21 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.