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    Rabbit anti-NCSTN Polyclonal Antibody

    Rabbit anti-NCSTN Polyclonal Antibody

    Catalog Number: abs135762 Reactivity: Human;Mouse;Rat Conjugation: Brand: Absin
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    Product Details

    Product Specification

    antigen NCSTN
    subcellular_location Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    Predicted Reactivity Rabbit
    Clonality Polyclonal Antibody
    Immunogen A synthesized peptide derived from human NCSTN.
    Species Reactivity Human;Mouse;Rat
    UniProt Q92542
    Purification The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
    Specificity NCSTN Antibody detects endogenous levels of total NCSTN.
    Synonym Anterior pharynx defective 2; APH 2; APH2; ATAG1874; KIAA0253; Ncstn; NCT; NICA_HUMAN; Nicastrin; RP11 517F10.1; RP11517F101;
    Post-translational modifications N-glycosylated.
    swiss_prot https://www.proteinatlas.org/Q92542
    Host Rabbit
    primary accession Q92542
    Form Liquid
    Description Nicastrin is a transmembrane glycoprotein serving as an essential component of the γ-secretase complex (1,2). Nicastrin is physically associated with presenilin and plays an important role in the stabilization and correct localization of presenilin to the membrane-bound γ-secretase complex (3). Nicastrin also serves as a docking site for γ-secretase substrates such as APP and Notch, directly binding to them and properly presenting them to γ-secretase to ensure the correct cleavage process (2,4).
    page_application WB;IHC;ELISA
    Storage Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
    tissuespecificity Detected in brain (at protein level) (PubMed:10993067). Widely expressed (PubMed:11396676).
    Background Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein) (PubMed:10993067, PubMed:12679784, PubMed:25043039, PubMed:26280335). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels.
    Concentration 1mg/ml
    Storage Temp. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
    Applications WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
    Cellular localization Endoplasmic reticulum;Extracellular region or secreted;Golgi apparatus;Lysosome;Plasma Membrane;