Flow cytometric analysis of C57BL/6 mouse splenocytes labeled with Rabbit IgG Isotype Control antibody at 1/50 dilution (1 μg) / (right panel) compared with an unlable Control / (left panel). Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Rabbit mAb IgG Isotype Control (S-4900)
Rabbit mAb IgG Isotype Control (S-4900)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Clone Number | S-4900 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | FCM, ICFCM |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Background
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
Picture
Picture
FC
Flow cytometric analysis of C57BL/6 mouse splenocytes fixed with 4% paraformaldehyde and permeabilized with 0.1% Tween. Cells were labeled with Rabbit IgG Isotype Control antibody at 1/50 dilution (1 μg) / (right panel) compared with an unlable Control / (left panel). Goat Anti- Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Total viable cells, as determined by Fixable Viability Dye 452 (S0B88801), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
